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Animal model of Pancreatic Cancer

Animal model of Pancreatic Cancer
胰腺癌动物模型
批准号：
8157681
负责人：
Syed Hussain
金额：
$37.46万
依托单位：
DIVISION OF BASIC SCIENCES - NCI
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/8157681
关键词：
Animal ModelMalignant neoplasm of pancreas

项目摘要

项目成果

Syed Hussain的其他基金

相关文献

中文摘要
翻译
我们建立了LSL-Kras G12 D（条件性K-ras突变体）、LSL p53 R172 H（条件性p53突变体）、Pdx-1-Cre（胰腺特异性Cre重组酶转基因）、NOS 2、IL-6和MIF基因敲除小鼠的克隆。我们还产生了NOS 2缺陷型LSL-Kras G12 D和LSL p53 R172 H小鼠。为了激活条件突变K-ras和p53等位基因，将这些小鼠与Pdx-1-Cre小鼠交配。将IL-6缺陷型小鼠与LSL-Kras G12 D（条件性K-ras突变体）杂交以产生IL-6缺陷型条件性K-ras突变体小鼠。激活后，Kras和p53双突变小鼠在3-6个月内发展PDAC。将这些小鼠与那些N 0 S2、MIF或IL-6无效的K-ras和p53双突变小鼠进行比较。
英文摘要
We have established the colonies of LSL-Kras G12D (conditional K-ras mutant), LSL p53 R172 H (conditional p53 mutant), Pdx-1-Cre (pancreas-specific Cre-recombinase transgenic), NOS2-, IL-6- and MIF-knockout mice. We have also generated NOS2-deficient LSL-Kras G12D and LSL p53 R172 H mice. To activate the conditional mutant K-ras and p53 alleles these mice are bred with Pdx-1-Cre mice. IL-6 deficient mice are crossed with LSL-Kras G12D (conditional K-ras mutant) to generate IL-6-deficient conditional K-ras mutant mice. Following activation, the Kras and p53 double mutant mice develops PDAC in 3-6 months. These mice will be compared with those K-ras and p53 double mutant mice that are null for NOS2, MIF or IL-6.
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Animal model of Pancreatic Cancer
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Forkhead-box (FOX) Transcription Factors in the Progression of Pancreatic Cancer