Shape change and Nitric Oxide (NO) Modulation of Core Pluripotent TF Expression

我们最初的假设可能仍然是正确的，但我们的重点在这一年转移到一个更公正的转录组学方法。我们对克隆A和CX-1的7天单层和球状体培养物中表达的基因进行了全转录组学分析。数据仍在评估中，但表明在克隆A和CX-1球状体中有462个基因上调，其中249个基因在克隆A中上调，但在CX-1中下调，其中1，195个基因在CX-1中上调，在克隆A中下调，1，861个基因在CX-1和克隆A中下调。这些变化来自参考序列列表中总共超过29，000个基因，并且添加了上一次refSeq构建中不存在的几种重要基因亚型。 我们现在正在确定哪些途径受影响最大。我们的重点是识别可能对治疗重要的途径，如细胞增殖和细胞死亡。

Our initial postulate may still be correct but our focus shifted during the year to a more unbiased transcriptomic approach. We have performed whole transcriptomic analysis of the genes expressed in 7 day monolayer and spheroid cultures of Clone A and CX-1. The data are still under evaluation but suggest that there are 462 genes upregulated in both Clone A and CX-1 spheroids with 249 genes up in Clone A but down in CX-1 with 1,195 genes up in CX-1 down in Clone A and 1,861 genes down in CX-1 and Clone A. These changes are out of a total of more than 29,000 genes in the reference sequence list and the addition of several important isoforms of genes that are not present in last refSeq build. We are now in the process of determining what pathways are most affected. Our focus is on identification of pathways such as cell proliferation and cell death that may be important for therapy.