Biomedical Research Core 2: Epigenetics Core

项目摘要 生物医学研究核心2：表观遗传学核心 (核心董事：李小刚；核心联席董事：肯尼斯·彼得森) 表观遗传学核心的目标是帮助PKD研究人员了解和研究 在PKD中转录和表观遗传调控被破坏的机制，并使用这一点 为制定具体有效的治疗策略提供信息。为此，表观遗传学核心将 为当地和扩展的PKD社区提供服务和试剂，以促进PKD的努力 研究人员在PKD科学这一令人兴奋的新前沿领域做出尖端发现。核心意志 以肾脏研究所和KUMC表观遗传学中心的现有资源和能力为基础 和干细胞生物学(由Kenneth Peterson博士执导)，以及 基因组测序设施和生物统计和信息学共享资源可在KUMC获得。这个 CORE将帮助PKD研究人员识别和研究PKD相关基因的表观遗传修饰 并研究调节与PKD相关的信号通路的表观遗传学机制。核心意志 为研究人员提供各种快速周转服务，包括DNA甲基化分析、染色质 免疫沉淀(CHIP)、芯片测序和实时定量PCR基因表达分析，以及 将提供关键表观遗传调控因子的敲除小鼠模型。这些服务和试剂将有所帮助 研究人员发现了PKD中破坏的表观遗传信号通路，并将有助于识别 开发新疗法的目标。核心中心还将帮助开展教育/咨询计划 为了帮助PKD研究人员更好地了解一般和PKD中的表观遗传学机制，并将提供 技术培训，帮助研究人员设计新的表观遗传学相关研究项目。

Project Abstract Biomedical Research Core 2: Epigenetics Core (Core Director: Xiaogang Li; Core Co-Director: Kenneth Peterson) The goal of the Epigenetics Core is to help PKD researchers understand and investigate the mechanisms by which transcriptional and epigenetic regulation are disrupted in PKD, and to use this information to develop specific and effective therapeutic strategies. To this end, the Epigenetics Core will provide services and reagents to the local and extended base PKD community to facilitate the efforts of PKD investigators to make cutting-edge discoveries in this new and exciting frontier in PKD science. The Core will build upon existing resources and capabilities in the Kidney Institute, and the KUMC Center for Epigenetics and Stem Cell Biology (Directed by Dr. Kenneth Peterson), together with outstanding capabilities of the Genome Sequencing Facility and the Biostatistics and Informatics Shared Resource available at KUMC. The Core will help PKD researchers to identify and investigate epigenetic modifications on PKD-associated genes and to examine epigenetic mechanisms that regulate signaling pathways associated with PKD. The Core will provide a variety of rapid-turnaround services to researchers, including DNA methylation analysis, chromatin immunoprecipitation (ChIP), ChIP-sequencing, and real-time quantitative PCR gene expression analysis, and will provide knockout mouse models of key epigenetic regulators. These services and reagents will help investigators identify epigenetic signaling pathways disrupted in PKD and will facilitate the identification of targets for the development of new therapies. The Core will also help with an education/consultation program to help PKD researchers better understand epigenetics mechanisms in general and in PKD, and will provide technical training to help investigators design new epigenetics-related research projects.