Core B: Molecular Detection and Stereology Core

分子检测和体视学核心(MDSC)为UAB神经科学界提供了成本- 在免疫组织化学(IHC)和原位杂交(ISH)各方面的有效培训和专业知识 应用于现代神经科学研究的程序。近十年来，Core一直专注于 培训技术人员、本科生和研究生、博士后研究员和教员调查人员 IHC和ISH的理论和实践。这种对教育和动手培训的重视促进了 这些技术的传播超越了核心本身，并允许将IHC和ISH集成到许多 NINDS资助的UAB实验室。对于有经验的用户，酷睿直接协助故障排除 疑难抗体，建立先进的检测放大技术，如酪胺信号 扩增和量子点，以及开发多标记检测程序。在这场竞争性的更新中 大卫·斯坦德尔特博士已经担任了B核心的首席研究员。他是一名临床医生- 长期致力于研究并在实验方面有出色背景的科学家 神经解剖学。特里·刘易斯博士仍然是Core的技术总监，拥有十多年的经验 与神经科学相关的研究。刘易斯博士和Marissa Menard女士，一位才华横溢的实验室技术人员，合作 在核心用户的培训和实验调查过程中与其密切和直接接触。现建议： 继续这些服务。 建议MDSC提供MicroBrightfield体视学/形态测量系统，该系统进一步 使UAB神经学家能够准确评估和量化他们的IHC和ISH的结果 调查。将在MDSC中提供的其他立体学服务将核心定位为 培训和协助UAB神经科学研究人员进行IHC和ISH的完整规划和执行 实验从组织固定、切片制备、IHC/ISH方案优化和最终分析 利用MicroBrightfield系统。 在过去九年的资助中，MDSC已被证明对NINDS资助的NINDS有很大的服务 调查人员和整个UAB神经科学界。核心持续培训和协助超过50人 每年的学生、博士后和研究人员，被广泛认为是IHC、ISH和 体视学专业知识..在当前申请的19项符合资格的赠款中，有16项利用了MDSC 强调核心的生产力和对其服务的明确持续需求。通过提供这些服务， MDSC减少了实验室的启动时间和多余资源，希望采用IHC、ISH和 将体视学方法融入他们的研究，并通过以下方式提高更有经验的研究人员的工作效率 扩大他们的能力，以实施最近开发的强大的新的灵敏检测方法。

The Molecular Detection and Stereology Core (MDSC) provides the UAB Neuroscience community with cost- effective training and expertise in all aspects of immunohistochemistry (IHC) and in situ hybridization (ISH) procedures as applied to modern neuroscience research. For nearly ten years, the Core has focused on training technicians, undergraduate and graduate students, post-doctoral fellows, and faculty investigators in the theory and practice of IHC and ISH. This emphasis on education and hands on training facilitates the propagation of these techniques beyond the Core itself and allows integration of IHC and ISH into many of the NINDS funded laboratories at UAB. For experienced users, the Core directly assists with troubleshooting difficult antibodies, establishing advanced detection amplification techniques, such as tyramide signal amplification and Quantum Dots, and developing multi-label detection procedures. In this competitive renewal application, Dr. David Standaert has assumed the role of Principal Investigator of Core B. He is a clinician- scientist with a longstanding commitment to research and an excellent background in experimental neuroanatomy. Dr. Terry Lewis remains the Technical Director of the Core and has over ten years' experience in neuroscience related research. Dr. Lewis and Ms. Marissa Menard, a talented laboratory technician, work closely and directly with Core users throughout their training and experimental investigations. It is proposed to continue these services. It is proposed that the MDSC provide MicroBrightField stereology/morphometric systems which further enables UAB neuroscientists to accurately assess and quantitate the results of their IHC and ISH investigations. The additional stereology services to be offered in the MDSC uniquely positions the Core to train and assist UAB Neuroscience investigators with complete planning and performance of IHC and ISH experiments from fixation of tissue, section preparation, IHC/ISH protocol optimization, and final analysis utilizing the MicroBrightField systems. Over the previous nine years of funding, the MDSC has proven to be of great service to NINDS funded investigators and the entire UAB Neuroscience community. The Core consistently trains and assists over 50 students, postdocs, and investigators per year and is widely recognized as the “go to” place for IHC, ISH and stereology expertise.. Of the 19 qualifying grants in the current application, 16 have utilized the MDSC emphasizing the Core's productivity and clear ongoing need for its services. By providing these services, the MDSC reduces startup time and redundant resources for laboratories wishing to incorporate IHC, ISH and stereology methods into their research as well as increasing productivity of more experienced investigators by expanding their capabilities to implement recently developed and powerful new sensitive detection methods.