Cell, Tissues, and Animal Core

instmctions）; 动物、细胞和组织培养核心（核心B）将负责向研究者提供 计划项目（PPG）与动物模型，组织和细胞，以实现其特定的目标。 该PPG的实验集中于识别负责以下之间的寿命差异的机制： 啮齿动物的种类。啮齿动物为这种寿命比较研究提供了一个独特的机会， 在同一个哺乳动物目中，寿命差异超过10倍。例如，短- 活的啮齿动物，如小鼠和大鼠，寿命为3-5年，而长寿的啮齿动物，如裸鼹鼠，失明 鼹鼠、灰松鼠、海狸和豪猪的最长寿命为21至32岁。到 为了研究这些动物长寿的分子机制，必须提供 来自多个啮齿动物物种的多个个体的生物材料。核心B将维持和补充， 根据需要，收集来自18种啮齿动物的组织和原代细胞。核心将提供 用组织、细胞、DNA和RNA进行项目研究，并对细胞进行诱变处理。在 的增订条文核心B将保留小鼠和裸鼹鼠供项目研究者使用。考虑 从非商业化的非标准微生物中收集和建立细胞培养物所需的努力 可用，集中资源将节省时间和精力，并降低成本！维护集中的 啮齿动物的收集也将标准化培养条件、质量控制和生物样品以供使用 在PPG项目中，提高结果的可重复性，并允许对相同的个体动物进行分析 通过几个分析和项目，以及尽量减少动物的使用。 相关性（参见说明）; 动物、细胞和组织培养核心（核心B）将维持动物、细胞和组织采集， 项目调查员。这一资源对于所有四个项目实现其具体目标至关重要。

instmctions); The Animal, Cell and Tissue Culture Core (Core B) will be responsible for providing investigators of the Program Project (PPG) with animal models, tissues and cells necessary to achieve their specific aims. Experiments of this PPG focus on identifying mechanisms responsible for differences in longevity between the species of rodents. Rodents offer a unique opportunity for such comparative study of longevity because of over 10-fold differences in life spans represented within a single mammalian order. For example, short- lived rodents, such as mouse and rat, live 3-5 years, while long-lived rodents, such as naked mole rat, blind mole rat, grey squirrel, beaver, and porcupine have maximum lifespans ranging from 21 to 32 years. To enable the study of molecular mechanisms of longevity in these animals, it is essential to provide access to biological materials from several individuals of multiple rodent species. Core B will maintain and replenish, as needed, the collection of tissues and primary cells from 18 species of rodents. The Core will provide project investigators with tissues, cells, DNA, and RNA, and perform mutagenic treatments of cells. In addition. Core B will maintain mice and naked mole rats for the use by project investigators. Considering the effort needed to collect and establish cell cultures from non-standard organisms that are not commercially available, the centralized resource will save time and effort, and reduce costs! Maintaining the centralized rodent collection will also standardize culture conditions, quality control and biological samples for use across PPG projects, improve reproducibility of results and allow the analysis of the same individual animals by several assays and projects, as well as minimize animal use. RELEVANCE (See instructions); The Animal, Cell and Tissue Culture Core (Core B) will maintain animal, cell and tissue collection for the project investigators. This resource is essential for all the four projects to achieve their specific aims.