Single round infection chikungunya virus as a new vaccine candidate

 描述（申请人提供）：基孔肯雅病毒（CHIKV）是人类疾病的重要病原体。最近，这种病毒已转移到西半球，并在加勒比群岛上广泛流行，并经常传入美国。在2013年至2014年的一年内，与当地传播有关的病例数从1例增加到近100万例，这表明我们正处于新世界大规模疫情爆发的开始。通常，CHIKV引起严重的自限性疾病，其特征在于发热、皮疹和剧烈的关节疼痛。此外，最近的流行病也表明CHIKV已变得更具毒性。以前的CHIKV传播与埃及伊蚊有关，但近年来，Ae.白纹伊蚊成为同样重要的CHIKV载体。这种蚊子在美国很常见，未来CHIKV的广泛传播似乎只是时间问题。尽管它们作为新兴病毒的公共卫生威胁和作为生物武器的应用潜力，但尚未开发出针对CHIKV感染的许可疫苗或治疗手段。该研究项目是基于疫苗设计新概念的进一步发展。它将导致产生假灭活的CHIKV（CHIK PIV），其在体内仅产生单轮感染。新的CHIKV候选疫苗将联合收割机活疫苗的效率和灭活或亚单位疫苗的安全性。

 DESCRIPTION (provided by applicant): Chikungunya virus (CHIKV) is an important etiologic agent of human disease. Recently, this virus has moved to Western hemisphere and has developed wide-spread epidemics on the Caribbean Islands with frequent introduction into US. Within one year, from 2013 to 2014, number of cases associated with local transmission increased from one to almost a million, suggesting that we are in the beginning of large outbreak in the New World. Typically, CHIKV causes a severe, self-limited disease characterized by fever, rash and excruciating joint pain. Moreover, the recent epidemics also suggested that CHIKV has become more virulent. Previous CHIKV transmission was associated with Aedes aegypti mosquitoes, but in the recent years, Ae. albopictus became an equally important vector of CHIKV. This mosquito species is common in the US, and future wide spread of CHIKV appears to be just a matter of time. Despite their public health threat as emerging virus and potential for application as biological weapons, no licensed vaccines or therapeutic means have been developed against CHIKV infection. This research project is based on further development of the new concept in vaccine designing. It will result in generation of pseudoinactivated CHIKV (CHIK PIV), which produces only a single round infection in vivo. New CHIKV vaccine candidate will combine the efficiency of live vaccines and safety of inactivated or subunit ones.