Mouse Model and Protein Expression Core

这三个项目都依赖于转基因小鼠和生产纯化蛋白和多克隆和 单克隆抗体。因此，所有三个项目都将得到老鼠模型和蛋白质的支持 Expression Core(核心A)，由Loren Fong博士领导。方博士是各种实用领域的专家。 细胞和分子生物学技术，包括培养和操纵小鼠胚胎干细胞 细胞和小鼠模型在生物医学研究中的应用。这种专业知识为此付出了巨大的回报 PPG。方博士与Reue、Young和Tontonoz博士合作，创造了常规和组织- 特定的Lipin2、Lipin3、Idol和Tle3基因敲除小鼠。此外，核心房屋和基因型鼠， 将小鼠回交到近交系，维护动物规程，并组织对 老鼠模型。 核心还生产用于生化研究的纯化蛋白质，以及用于产生多克隆和 单抗；这是一个基本的功能，因为我们的PPG专注于新发现的 分子，这些分子的试剂很少。在之前的资助期间，Bensadoun博士、Fong和Young博士 为这种PPG生产了多种抗体试剂。他们的专业知识和经验将是新员工的福音 本申请中提出的抗体项目。除了抗体试剂，Core还将生产 用于生化和结构生物学研究的纯化重组蛋白。PPG的所有三个项目 将使用核心A。 相关性(请参阅说明)： 该计划项目赠款建议定义调节脂质代谢的分子机制和 肥胖-在代谢性疾病发病机制中起中心作用的两个事件(例如，顺从和 动脉粥样硬化)。小鼠模型和蛋白质表达核心将通过提供 所有三个项目都有新的小鼠模型、抗体和重组蛋白。

All three projects rely on genetically modified mice and the production of purified proteins and polyclonal and monoclonal antibodies. Accordingly, all three projects will be supported by the Mouse Model and Protein Expression Core (Core A), led by Dr. Loren Fong. Dr. Fong is an expert in a wide variety of practical techniques in cellular and molecular biology, including growing and manipulating mouse embryonic stem cells and the use of mouse models in biomedical research. This expertise has paid huge dividends for this PPG. Dr. Fong, working with Drs. Reue, Young, and Tontonoz, has generated conventional and tissue- specific Lipin2, Lipin3, Idol, and Tle3 knockout mice. In addition, the Core houses and genotypes mice, backcrosses mice onto inbred strains, maintains animal protocols, and organizes histological studies on mouse models. The Core also produces purified proteins for biochemical studies and for generating polyclonal and monoclonal antibodies; this has been an essential function, since our PPG has focused on newly discovered molecules, for which few reagents exist. During the prior funding period, Drs. Bensadoun, Fong and Young produced multiple antibody reagents for this PPG. Their expertise and experience will be a boon for the new antibody projects proposed in this application. Aside from antibody reagents, the Core will also produce purified recombinant proteins for biochemical and structural biology studies. All three projects of the PPG will use Core A. RELEVANCE (See instructions): This Program Project Grant proposes to define the molecular mechanisms that regulate lipid metabolism and adiposity-two events that play centrol roles in the pathogenesis of metablic disesase (e.g., obeisity and atherosclerosis). The Mouse Model and Protein Expression Core will support the PPG's goals by providing new mouse models, antibodies, and recombinant proteins to all three projects.