Exploring Design Principles of Cellular Control Circuits

一、总体主管PI：温德尔·林 探索蜂窝控制电路的设计原理 摘要 加州大学旧金山分校系统和合成生物学中心专注于细胞如何 使用基因编码的分子网络做出决定。我们的理念是结合使用 网络工程、分析和建模，全面了解底层算法 细胞用来做出监管决策的原则。因此，我们的中心专注于开发新的 用于网络扰动和重新布线的合成生物学工具，以及用于 分析这些变化如何改变细胞行为。这些工具结合在一起，将使我们能够更好地绘制基因图谱- 控制细胞动态和空间反应的表型关系。对于第二笔资金 在此期间，我们的中心正将其大部分创新努力集中在试图应用我们对细胞决策的理解上- 走向医学：我们最具前瞻性的目标是使用合成生物学方法来设计细胞， 可用于治疗，包括免疫细胞和具有定制用户定义反应的干细胞 行为。设计的治疗性细胞为我们理解细胞逻辑提供了最终的试验台。更多 总的来说，该中心的目标是在这一变革性领域的发展中发挥领导和促进作用， 无论是在我们自己的机构还是在国家和国际科学和教育界。

I. OVERALL Lead PI: Wendell Lim Exploring Design Principles of Cellular Control Circuits SUMMARY The UCSF Center for Systems and Synthetic Biology is focused on the fundamental question of how cells make decisions using genetically encoded molecular networks. Our philosophy is to use a combination of network engineering, analysis, and modeling to comprehensively understand the underlying algorithmic principles that cells use to make regulatory decisions. Our Center is therefore focused on developing new synthetic biology tools for network perturbation and rewiring, as well as quantitative, single-cell approaches for analyzing how such changes alter cellular behavior. Together such tools will allow us to better map genotype- phenotype relationships that control both dynamic and spatial responses in cells. For the second funding period, our Center is focusing much of its innovative effort on trying to apply our understanding of cell decision- making to medicine: our most forward-looking aim is to use synthetic biology approaches to engineer cells that could be used as therapeutics, including immune cells and stem cells with customized user-defined response behaviors. Designer therapeutic cells provide the ultimate testbed for our understanding of cellular logic. More broadly, the Center aims to play a leadership and catalytic role in the development of this transformative field, both at our own institution and within the national and international scientific and educational community.