Direct Deposition of Polymer Fibers for Use as a Surgical Sealant

 描述(申请人提供)：组织重建和缝合几乎所有的手术干预和创伤性损伤的伤口和伤口。该项目旨在开发一种医用密封剂，在降低成本、炎症反应和疾病传播风险的同时，改善程序结果。目前的临床选择受到成本高、材料性能差和生物兼容性问题的综合影响。利用一种名为溶液吹风纺丝的技术，聚合物纤维可以直接沉积在任何表面上。溶液吹塑纺丝有可能成为外科和生物材料制造中的一种强有力的工具。本提案的总体目标是研究溶液吹塑聚合物共混物聚乙二醇(PEG)和聚乳酸-乙醇酸(PLGA)在肠吻合口模型中作为外科密封剂的应用。这项研究的假设是：溶液吹气纺丝的PLGA/PEG共混物将比现有的密封剂策略具有优势，导致更好的外科手术结果。这项建议旨在利用马里兰大学功能高分子实验室(UMD)和谢赫·扎耶德儿童国家儿科外科创新研究所的互补专业知识和设施。建议研究的具体目标是：1.粘接剂的优化、表征和体外评估2.聚合物功能化以改善粘附性和体外表征3.吹塑聚合物密封剂在急、慢性临床前模型中的体内临床前疗效和生物相容性。这项工作旨在将材料特性和沉积条件与体外和体内的疗效相关联，并将导致对有效外科材料的开发有更深入的了解。最终，使用溶液吹塑纺丝在任何表面上直接沉积聚合物纤维结构的成功开发将导致在各种外科应用和生物材料制造中的临床可翻译方法。

 DESCRIPTION (provided by applicant): Tissue reconstruction and closure of incisions and wounds is pertinent to almost all surgical interventions and traumatic injuries. This project aims to develop a medical sealant that reduces cost, inflammatory response and disease transmission risk, while improving procedural outcomes. Current clinical options suffer from a combination of high cost, poor material properties, and biocompatibility issues. Utilizing a technique called solution blow spinning, polymer fibers can be deposited directly onto any surface. Solution blow spinning has the potential to be a powerful tool in surgery and in biomaterials fabrication. The overall objective of this proposal is to investigate the use of solution blow spun polymer blends of poly(ethylene glycol) (PEG) and poly(lactic-co-glycolic acid) (PLGA) for use as a surgical sealant in an intestinal anastomosis model. The hypothesis for this research is: solution blow spun PLGA/PEG blends will have advantages over existing sealant strategies, leading to better surgical procedure outcomes. This proposal has been designed to utilize the complementary expertise and facilities at the University of Maryland's Functional Macromolecular Laboratory (UMD) and the Sheikh Zayed Institute for Pediatric Surgical Innovation at Children's National. The Specific Aims of the proposed research are: 1. Adhesive optimization, characterization, and in vitro assessment 2. Polymer functionalization for improved adhesion and in vitro characterization 3. In vivo pre- clinical efficacy and biocompatibility of blow spun polymer sealants in acute and chronic pre-clinical models. This work aims to correlate material properties and deposition conditions to in vitro and in vivo efficacy, and will lead to greater insight into the development of effective surgical materials. Ultimately, successful development of the direct deposition of polymer fiber constructs using solution blow spinning onto any surface could lead to clinically translatable approaches in a diverse variety of surgical applications and in biomaterials fabrication.