Rutgers Mass Spectrometry Center for Integrative Neuroscience Research

 描述（由申请人提供）：NINDS P30计划的先前资金支持UMDNJ的神经蛋白质组学核心设施。随着罗格斯大学和UMDNJ的整合，我们现在请求支持扩大罗格斯质谱中心的综合神经科学研究。罗格斯大学在神经科学方面有着杰出的深度，在过去的资助期间，核心通过为NINDS研究人员提供先进的蛋白质组学技术，并作为合作和新技术开发的中心，成功地推进了神经科学研究和教育任务。 在此更新申请中，我们将为NINDS资助的PI提供当地最先进的蛋白质组学技术，并继续升级我们的核心设备。Scientific Cores将位于罗格斯大学的纽瓦克和新玩法/皮斯卡特维校区，分别由联合PI的Hong Li博士和Peter Lobel博士领导，Hong Li博士在管理NeuroProteomics Core方面拥有10多年的经验，Peter Lobel博士在神经科学研究中使用蛋白质组学方面拥有丰富的经验。两个核心的专业知识和技术是互补的，但两个地点的一个关键理由是NINDS研究人员将能够在当地访问核心。这将允许研究人员和机构工作人员之间的互动，这对于MS研究的成功规划，执行和解释至关重要。NIND研究人员的技术能力将得到扩展，可以使用两台新的Thermo Q Exactive混合四极杆轨道阱质谱仪。这是一种高灵敏度/高通量仪器，非常适用于蛋白质鉴定， 蛋白质翻译后修饰的定量以及生物标志物的发现和验证。 罗格斯大学承诺为核心提供出色的机构承诺，包括现代化的实验室空间和PI和行政支持人员的工资支持。由NINDS和其他调查人员组成的两个委员会将监督该设施的财务和科学管理以及日常运作。用户俱乐部将为教育、培训、数据共享和协作提供一个重要论坛。正如我们过去的成功记录所证明的那样，这一扩大的核心将保持一个健全的科学和财务管理结构，并有效地用于提高NINDS赞助研究的研究能力。

 DESCRIPTION (provided by applicant): Previous funding from the NINDS P30 Program has supported the NeuroProteomics Core Facility of UMDNJ. With the integration of Rutgers University and UMDNJ, we now request support for an expanded Rutgers Mass Spectrometry Center for Integrative Neuroscience Research. Rutgers has outstanding depth in neuroscience with over 350 researchers in this area and in the past funding period, the Core successfully advanced neuroscience research and educational missions by providing NINDS researchers access to advanced proteomics technologies, and serving as a hub for collaborations and new technology developments. In this renewal application, we will provide NINDS-funded PI's with local access to state-of-the-art proteomics technology with the continued upgrade our Core equipment. Scientific Cores will be located on the Newark and New Brunswick/Piscataway campuses of Rutgers University and respectively directed by Co-PI's Dr. Hong Li, who has over 10 years' experience in managing the NeuroProteomics Core and Dr. Peter Lobel, who has extensive experience in using proteomics in neuroscience research. Expertise and technology of the two cores are complementary, but a key rationale for two locations is that NINDS researchers will be able to access the Core locally. This will allow interaction between researchers and facility staff that is critical for the successful planning, execution and interpretation of MS studies. Technical capabilities will be expanded for NINDs researchers with access to two new Thermo Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometers. This is a high-sensitivity/high-throughput instrument that is highly applicable for protein identification, quantification of protein post-translational modifications and biomarker discovery and validation. Rutgers has promised excellent institutional commitment for the Core including modern laboratory space and salary support for both PIs and administrative supporting personnel. Two committees, composed of NINDS and other investigators, will oversee the facility's fiscal and scientific management as well as its daily operation. A User Club will provide an important forum for education, training, data sharing and collaborations. As demonstrated by our past record of success, this expanded Core will be maintained with a sound scientific and financial management structure and effectively utilized to enhance research capabilities for NINDS-sponsored research.