Gel documentation system

凝胶记录系统是我们研究的核心设备，电泳凝胶和薄层色谱通常需要查看，记录和分析。在Daayf博士的实验室中，研究了控制植物防御抑制的分子和生化机制，使目前主要的病原菌菌株能够克服马铃薯的防御。这是通过研究大肠杆菌葡聚糖与肽抑制因子在防御基因下调中的作用来完成的。在植物方面，学生们将研究是否pal和hmg的其他同工异构体在响应病原菌抑制因子时，以牺牲抑制基因为代价积累。这些研究包括使用dna - aflp，减法杂交，以及肽的提取和分离。这项研究对于了解最具攻击性的病原菌是如何打败马铃薯的防御反应具有重要意义，并有望为更有效的基于知识的疾病控制策略奠定基础。Stasolla博士还将使用这些设备来研究体细胞胚胎发生的不同分子方面，Razem博士将使用这些设备来研究控制基因表达的调节机制，特别是那些涉及脱落酸的机制。

The Gel Documentation System is a piece of equipment that is central to our studies where electrophoresis gels as well as thin layer chromatograms routinely need to be viewed, documented, and analyzed. In Dr. Daayf's laboratory, the molecular and biochemical mechanisms governing the plant defense suppression that allows the currently predominant strain of P. infestans to overcome potato defenses are studied. This is being completed by studying the involvement of P. infestans glucans versus peptidic suppressors in defense gene downregulation. On the plant side, students will investigate whether other isoforms of pal and hmg accumulate at the expense of the suppressed gene in response to P. infestans suppressors. These studies involve the use of cDNA-AFLP, subtractive hybridization, as well as peptides extraction and separation. This research is important in order to understand how the most aggressive strains of P. infestans defeat potato defense reactions, and hopefully will lay the groundwork for more efficient knowledge-based disease control strategies. The requested equipment will be also used by Dr. Stasolla to study different molecular aspects of somatic embryogenesis, and Dr. Razem to study regulatory mechanisms that govern gene expression, in particular those involving abscissic acid.