Application for a HPLC purification system

申请的目的是申请资金，用于购买带有梯度泵、UV-Vis检测器和折射率检测器的高效液相色谱系统。该系统设计有两个进样器，能够处理分析型和半制备型样品。在我的NSERC发现基金中提出了三个主题。第一个主题是合理设计和合成肿瘤相关糖基转移酶的碳水化合物抑制剂；第二个主题是研究一类寡唾液酸，并利用它们来制备高亲和力的抗体，这些抗体能够通过与末端残基结合来识别天然的多唾液酸抗原；第三个主题是开发一种通用而有效的碳水化合物抗原聚集策略，通过引入B细胞受体和合成的簇分子之间的多价相互作用来提高免疫原性。我们的研究将常规地涉及重有机合成，并处理复杂的分子，如受保护和去保护的寡糖及其蛋白质结合物，众所周知，这些分子极难通过重结晶纯化。因此，能够处理各种有机和生物有机材料的高效液相色谱对我们的成功至关重要。

The application is to request funds for the purchase of a high performance liquid chromatography (HPLC) system with a gradient pump, a UV-Vis dectector and a refractive index detector. The system is designed with two injectors and is capable of handling both analytical and semi-preparative samples. Three themes have been proposed in my NSERC discovery grant. The first concerns the rational design and synthesis of carbohydrate inhibitors for tumour-associated glycosyltransferases; the second is to study a family of oligosialic acids and use them to raise high-affinity antibodies that are capable of recongnizing the natural polysialic acids antigens via binding to the terminus residues; the third theme is to develop a general and efficient clustering strategy for carbohydrate antigens to increase immunogeneicity through the introduction of multivalent interactions between B-cell receptors and the synthesized cluster molecules. Our research will routinely involve heavy organic syntheses and deal with complex molecules such as protected and de-protected oligosaccharides and their protein conjugates which are well-known to be extremely difficult to purify by recrystallization. A HPLC capable of handling various organic and bio-organic materials is thus essential for our success.