A Preparative HPLC Instrument for the Purification of Small Molecules

为了提纯有机分子，需要一套制备型高压液体净化系统。制备高效液相色谱是最有效的纯化方法之一，可以对高达数百毫克的分子进行纯化。目前合成有机化学家的最佳实践要求对纯度达到95%的产品进行纯化。该系统将允许各种不同类型的分子的纯化，包括杂环、生物碱、多肽、脱脂肽、大环、萜类、多肽仿造物以及多肽小分子和其他生物结合物。该系统将允许对具有挑战性的样品进行快速纯化，否则不可能或很难使用传统方法(如快速柱层析)进行纯化。此外，在进行生物检测之前，通常需要进行高效液相色谱柱纯化。拟议的高效液相系统将对我们解决合成和药物化学问题和项目的能力产生革命性的影响，否则这些问题和项目是不可能的。*拟议的高效液相系统将被用作“临时”仪器，主要服务于来自两个研究小组的学生和博士后研究人员的需求。然而，该系统将向多伦多大学化学系的其他用户提供。在有机化学领域受过培训的学生受到制药、生物技术和材料行业雇主的高度追捧。因此，这一主要仪器将为HQP提供宝贵的培训经验，包括合成有机化学或药物化学培训。**

A preparative high-pressure liquid purification (HPLC) system is requested for the purification of organic molecules. Preparative HPLC is one of the most powerful purification methods, and is amenable to the purification of molecules on scales up to hundreds of milligrams.******Current best practice for synthetic organic chemists requires purification of products with purities of 95%. The proposed system will allow for the purification of a variety of different molecular types, including heterocycles, alkaloids, peptides, depsipeptides, macrocycles, terpenoids, peptidomimetics, and peptide small-molecule and other bioconjugates. The system will allow for rapid purification of challenging samples that would otherwise be impossible or very difficult to purify using conventional methods such as flash column chromatography. Moreover, HPLC purification is often required as a final step before biological testing. The proposed HPLC system will have a transformative effect on our ability to tackle synthetic and medicinal chemistry problems and projects that would otherwise not be possible.******The proposed HPLC system will be utilized as a “walk-on” instrument and will primarily service the needs of students and post-doctoral researchers from two research groups. However, the system will be made available to other users in the Department of Chemistry at the University of Toronto. Students trained in the area of organic chemistry are highly sought after by employers in the pharmaceutical, biotech, and materials sectors. As such, this workhorse instrument will provide invaluable training experience for HQP with synthetic organic or medicinal chemistry training.**