A Preparative HPLC Instrument for the Purification of Small Molecules

用于纯化小分子的制备型 HPLC 仪器

• 批准号: RTI-2020-00710
• 负责人: Batey, Robert
• 金额: $ 8.02万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=692964
• 关键词: Preparative; HPLC; Instrument; Purification; Small

A preparative high-pressure liquid purification (HPLC) system is requested for the purification of organic molecules. Preparative HPLC is one of the most powerful purification methods, and is amenable to the purification of molecules on scales up to hundreds of milligrams.******Current best practice for synthetic organic chemists requires purification of products with purities of 95%. The proposed system will allow for the purification of a variety of different molecular types, including heterocycles, alkaloids, peptides, depsipeptides, macrocycles, terpenoids, peptidomimetics, and peptide small-molecule and other bioconjugates. The system will allow for rapid purification of challenging samples that would otherwise be impossible or very difficult to purify using conventional methods such as flash column chromatography. Moreover, HPLC purification is often required as a final step before biological testing. The proposed HPLC system will have a transformative effect on our ability to tackle synthetic and medicinal chemistry problems and projects that would otherwise not be possible.******The proposed HPLC system will be utilized as a “walk-on” instrument and will primarily service the needs of students and post-doctoral researchers from two research groups. However, the system will be made available to other users in the Department of Chemistry at the University of Toronto. Students trained in the area of organic chemistry are highly sought after by employers in the pharmaceutical, biotech, and materials sectors. As such, this workhorse instrument will provide invaluable training experience for HQP with synthetic organic or medicinal chemistry training.**

制备型高压液体纯化（HPLC）系统被要求用于有机分子的纯化。反相HPLC是最强大的纯化方法之一，适用于高达数百毫克的分子纯化。*目前合成有机化学家的最佳实践要求产品纯度达到95%。所提出的系统将允许纯化各种不同的分子类型，包括杂环、生物碱、肽、缩酚肽、大环、萜类化合物、肽模拟物和肽小分子和其他生物缀合物。该系统将允许快速纯化具有挑战性的样品，否则使用常规方法如快速柱色谱法是不可能或非常难以纯化的。此外，HPLC纯化通常需要作为生物测试之前的最后步骤。拟议的HPLC系统将对我们解决合成和药物化学问题的能力产生变革性影响，否则将不可能实现。拟议的HPLC系统将被用作“步行”仪器，主要服务于两个研究小组的学生和博士后研究人员的需求。不过，该系统将提供给多伦多大学化学系的其他用户。在有机化学领域接受培训的学生受到制药，生物技术和材料部门雇主的高度追捧。 因此，该主力仪器将为HQP提供合成有机或药物化学培训的宝贵培训经验。**