Upgrade of HPLC-DAD instrument for metabolic analysis

该申请要求提供资金，用于升级仪器（HPLC），以便能够检测和定量极少量的生物活性物质，否则无法确定。新的模块是必不可少的，使该仪器能够24小时/7天不间断地正常工作。大量的植物疾病是由称为真菌的微生物病原体引起的，它们在全世界造成最大的作物损失。为了寻找目前使用的杀菌剂的替代品，我们研究计划的长期目标是使用环境可接受的策略来了解和控制植物真菌病害。在我们的研究中特别强调的是一些最重要的加拿大油籽作物，油菜和油菜籽，以及蔬菜，如花椰菜和最具破坏性的真菌病原体。为了能够进行这项研究，我们正在培养新一代的生物有机化学家，他们可以使用生物和化学方法。化学方法涉及使用非常精确的技术来检测和鉴定极少量的生物活性物质。HPLC就是这样的仪器之一，在我们分析各种植物及其病原体的生物产品的工作中绝对至关重要。 该设备可供多名研究人员在未来多年内有效使用，无需进一步升级或大量维护成本。如果我们的研究能够有助于保护农作物和促进可持续农业实践，那么农业产业和生活在农业地区或非农业地区的每个人都将受益。

This application requests funds for upgrading an instrument (HPLC) that will allow the detection and quantification of extremely small amounts of bioactive substances, not possible to determine otherwise. The new modules are essential to allow this instrument to work routinely 24 h/7 days without interruption. A large number of plant diseases are caused by microbial pathogens called fungi that bring about the largest crop losses worldwide. To seek alternatives to the current use of fungicides, the long term objective of our research program is to understand and control plant fungal diseases using environmentally acceptable strategies. Of particular emphasis in our studies are some of the most important Canadian oilseed crops, canola and rapeseed, as well as vegetables such as cauliflower and their most damaging fungal pathogens. To be able to carry out this research we are training a new generation of bioorganic chemists who can use both biological and chemical methods. The chemical methods involve the use of very precise techniques for detection and identification of extremely small amounts of bioactive substances. The HPLC is one of such instruments, absolutely crucial in our work to analyze the bioproducts of diverse plants and their pathogens.  This equipment can be efficiently used by multiple researchers for many years to come without requiring further upgrade or significant maintenance costs. The farming industry and everybody, living in farming areas or not, will benefit if our research can contribute to protect agricultural crops and facilitate sustainable agriculture practices.