An automated benchtop flow cytometry system for multicolour cell analysis

这项应用是用于台式分析型流式细胞仪，将由分子和细胞生物学系的八名研究人员使用。所有申请者都得到了NSERC发现研究基金的资助，并参与了研究真核细胞的细胞和分子生物学的研究项目。流式细胞术是一种用于分析悬浮在流体中的细胞或颗粒的技术。流式细胞术已经成为细胞生物学家的重要工具，可以定量分析许多细胞特性，包括细胞活力、增殖、细胞表面和细胞内抗原、细胞内pH、钙和膜电位。技术进步降低了仪器成本，简化了仪器操作。由于校园内使用这种设备的机会有限，申请者对这种技术的使用受到了阻碍。系内迫切需要一种既易于操作又能灵活适应多用户研究环境需求的流式细胞仪。

This application is for a benchtop analytical flow cytometer that will be used by a group of eight researchers within the Department of Molecular and Cellular Biology. All of the applicants are funded by NSERC Discovery research grants and are engaged in research programs studying the cell and molecular biology of eukaryotic cells. Flow cytometry is a technique that is used for analyzing cells or particles suspended in a stream of fluid. Flow cytometry has become an essential tool for the cell biologist, allowing quantitative analysis of numerous cellular properties including cell viability, proliferation, cell surface and intracellular antigens, intracellular pH, calcium and membrane potential. Technological advances have reduced instrument costs and simplified their operation. The use of this technology by the applicants is hampered by limited access to this equipment on campus. There is an urgent need for a flow cytometer within the department that is both easy to operate and offers the flexibility to accommodate the demands of a multi-user research environment.