Characterization of mast cell basophil haematopoesis in patients with asthma and drug allergies.

这项研究的主要重点是分析血液中被认为与过敏性疾病有关的特定细胞，这些细胞来自对药物发生过敏反应的患者以及哮喘和其他过敏患者。这些患者循环中的某些细胞被认为与哮喘和其他过敏患者的临床表现有关。这些细胞具有某些特征，我们的实验室首次在哮喘患者的循环中鉴定出一种特定的细胞，我们认为这种细胞在这种疾病的发病机制中可能很重要。我们建议在哮喘患者中检查这些细胞，那些经历药物过敏反应和其他过敏反应的患者，并确定它们的特性及其对许多生长因子的生长反应，这些生长因子已被证明会影响试管中称为肥大细胞的细胞的生长。一旦我们确定了这些细胞所含的特定蛋白质和其他产物，我们将尝试将细胞的数量和类型与患者的临床过敏状态相关联。将从急性哮喘患者和缓解期患者中采集血液，以确定这些细胞与急性过敏反应是否存在任何特定关系。我们还将研究这些细胞如何调节体内的其他生理系统，特别是为什么患有急性过敏反应的患者血压显着下降的可能解释。我们可以用我们在初步研究中使用的特异性抗体来识别这些细胞。该抗体结合这些细胞的表面，然而该抗体结合的特异性蛋白质尚未被鉴定。我们建议使用分子生物学技术来鉴定这种蛋白质。这些研究将为我们提供关于这些细胞性质的重要见解，并可能导致新的治疗方法。

The major emphasis of this investigation is to analyse blood for specific cells thought to be implicated in allergic disease from patients undergoing allergic reactions to medications and in patients with asthma and other allergies. Certain cells in the circulation of these patients are thought to be involved in the clinical manifestation seen in patients with asthma and other allergies. These cells have certain characteristics and for the first time our laboratory has identified a particular cell in the circulation of patients with asthma which we believe may be important in the pathogenesis of this condition. We propose to examine these cells in patients with asthma, those experiencing allergic reactions to drugs and other allergies and determine their characteristics and their growth responses to a number of growth factors which have been demonstrated to affect the growth of cells known as mast cells in the test tube. Once we have characterised these cells in terms of what particular proteins and other products they contain we will then try to correlate the number and type of cell with the clinical allergic state of the patient. Blood will be obtained from patients undergoing acute asthma and those in remission to determine if there is any specific relationship of these cells with acute allergic reactions. We will be also looking at how these cells regulate other physiological systems in the body, in particular possible explanations why patients that suffer acute allergic reactions develop significant drops in blood pressure. We can identify these cells with a specific antibody that we have used in our preliminary studies. This antibody binds the surface of these cells, however the specific protein to which this antibody binds has not been identified. We propose to identify this protein by using molecular biology techniques. These studies will provide us with important insights on the nature of these cells and may lead to new therapies.