Anaerobic chamber system for culturing and characterizing novel anaerobic microorganisms from unique ecosystems

厌氧室能够安全地隔离、培养和检查需要无氧环境的厌氧微生物。所要求的厌氧室将支持Whyte、Bede和Driscoll博士的研究小组对厌氧微生物的分离、鉴定和鉴定。怀特的研究团队使用经典微生物学和新的基于基因组学的分子技术，研究了加拿大北极高原独特生境(永久冻土/地面冰、冷盐泉、微生物海冰垫)中的微生物生物多样性和生态。正在进行的独立培养研究表明，这些环境中含有与产甲烷菌、厌氧甲烷氧化细菌、硫酸盐还原细菌(SRB)和厌氧嗜盐古细菌相关的新的厌氧16S rRNA种型。怀特的实验室将使用厌氧室从这些环境中分离和鉴定厌氧菌株，并对永久冻土和盐泉样本进行厌氧活性分析。这将使我们能够定义厌氧微生物在超低温下的活动和生长，并使我们更好地了解这种嗜冷厌氧微生物在这些低温环境，特别是永久冻土中的营养循环和生物地球化学过程中所发挥的潜在作用。Bede对植物与昆虫的相互作用感兴趣，特别是了解植物防御反应的调节，以昆虫食草动物为目标。这涉及到对食草动物的识别，这是由昆虫口腔分泌物中的激发子介导的。最近，有研究表明，细菌肠道菌群影响毛虫反流物中的激发子，从而影响植物防御反应。比德的实验室将使用厌氧室来分离和鉴定毛虫肠道中的厌氧细菌，并确定它们是否可以催化形成脂肪酸-氨基酸激发子。Driscoll的研究小组研究了共生固氮，这是紫花苜蓿根瘤菌-紫花苜蓿系统中的一种厌氧生化过程。利用厌氧生长室，Driscoll的研究小组可以在厌氧环境中研究豆科植物的根瘤和类杆菌，这对一些生理研究是最佳的。

Anaerobic chambers enable the safe isolation, incubation and examination of anaerobic microorganisms which require oxygen-free environments. The anaerobic chamber requested will support the isolation, identification and characterization  of anaerobic microorganisms by the research groups of Drs. Whyte, Bede, and Driscoll. Whyte's research team examines microbial biodiversity and ecology in unique habitats (permafrost/ground ice, cold saline springs, microbial sea-ice matts) in the Canadian high Arctic using both classical microbiology and novel genomics-based molecular techniques. Ongoing culture independent studies have indicated that these environments harbour novel anaerobic 16S rRNA phylotypes related to methanogens, anaerobic methane oxidizing bacteria, sulphate reducing bacteria (SRB), and anaerobic halophilic Archaea. Whyte's lab would use the anaerobic chamber to isolate and characterize anaerobic strains from these environments as well as conduct anaerobic activity assays with permafrost and saline spring samples. This will enable us to define anaerobic microbial activity and growth at ultra-low temperatures and lead to a greater understanding of the potential roles such psychrophilic anaerobic microorganisms play in nutrient cycling and biogeochemical processes in these cryoenvironments, particularly permafrost. Bede is interested in plant-insect interactions, specifically in understanding the regulation of plant defense responses to target the insect herbivore. This involves the recognition of the herbivore, which is mediated by elicitors in the insect oral secretions. Recently, it has been shown that the bacterial gut flora effects the elicitors in the caterpillar regurgitant and, hence, plant defense responses. Bede's laboratory would use the anaerobic chamber to in isolate and identifying anaerobic bacteria in the caterpillar gut and determining whether they can catalyze the formation of fatty acid-amino acid elicitors. Driscoll's research group studies symbiotic nitrogen fixation, an anaerobic biochemical process in the Sinorhizobium meliloti - alfalfa system.  An anaerobic growth chamber will allow Driscoll's group to study legume root nodules and bacteroids in an anaerobic environment, as is optimal for some physiological studies.