Comparative genomics of fungi

真菌在有机物降解中起着重要作用，作为动植物疾病的病原体，在工业发酵中也具有重要的经济意义。真菌通常是高等真核生物中基因组大小最小的。因此，真菌的基因组测序比动物或植物的基因组多，到目前为止已公开的真菌基因组超过45个。真菌基因组测序和先进的注释提供了一个数据丰富的资源，易于访问。我建议使用在线来源的遗传数据，并辅之以我的研究计划中实验获得的数据，来研究真菌和其他一些有前途的微生物真核生物的生物学和进化。这项工作的目的是通过从测序的基因组中寻找可能在不同水平上进行系统发育分析的新的基因序列，并用湿实验室的工作补充现有的序列和数据，来研究真菌、粘菌和其他微生物真核生物的进化关系。这些目标是研究真菌进化和生物学的长期计划的一部分，使用比较基因组学结合基本测序和序列分析技术的新工具。真菌的真核特性和较小的基因组大小使其在比较基因组学中特别有价值，随着序列数据量的指数级增长，现在有可能访问和分析这些数据，以带来具有生物学意义的重要发现。

Fungi are of primary importance in organic matter degradation, as well as having major economic significance as agents of diseases of plants and animals, and in industrial fermentation. Fungi generally have the smallest genome sizes among higher eukaryotes.  Consequently, there are more genomes of fungi sequenced than those of animals or plants, with over 45 fungal genomes publicly available to date. The fungal genomes sequenced and the advanced annotation provide a data-rich resource with easy accessibility. I propose to examine the biology and evolution of fungi and some other promising microbial eukaryotes such as slime molds (myxomycota) using genetic data from online sources supplemented by data obtained experimentally in my research program. The purpose of this work is to investigate the evolutionary relationships of fungi, myxomycota and other microbial eukaryotes by searching for new gene sequences with or without annotation from sequenced genomes which may be useful in phylogenetic analyses at various levels, and supplementing the existing sequences and data with wet lab work. These objectives are part of a long term program investigating the evolution and biology of fungi, using the new tools of comparative genomics combined with basic sequencing and sequence analysis techniques. Their eukaryotic nature and small genome sizes make fungi particularly valuable in comparative genomics, and, with the exponentially increasing amounts of sequence data, it is now possible to access and analyse these data to bring forth important discoveries of biological significance.