Multi-user digital imaging system

我们的五个研究小组正在请求购买一台计算机化的多用途成像仪，该成像仪将使我们能够捕获来自一系列分子生物学应用的各种实验结果的数字化图像。成像器由一个封闭在光密室中的CCD摄像机组成，灯箱上方的灯箱可以转换为白光或紫外光。蛋白质、DNA和RNA在凝胶中溶解的图像可以在小室中染色和适当照明后获得。相机的高分辨率和灵敏度与波长选择滤光片相结合，使我们能够捕获和准确量化微弱和明亮的信号，使我们能够进行以前使用老式相机无法实现的应用。请求使用该成像仪的五个小组正在进行的研究跨越了一系列基础科学调查，包括：确定单个细胞膜跨越蛋白质的结构，剖析酶的结构与功能的关系，确定在细胞核中复制的寄生DNA元件如何借用宿主功能以确保其自身的维护，了解染色体DNA的包装如何影响基因的表达，以及比较不同脂肪酸对脂代谢的影响及其对动脉粥样硬化的影响。除了在本申请中被点名的研究小组外，该成像仪还将向其他研究小组开放，从而促进许多人的研究。

Our five research groups are requesting the acquisition of a computerized multi-purpose imager that will allow us to capture digitized images of a wide variety of experimental results from a range of molecular biology applications. The imager consists of a CCD camera enclosed in a light-tight chamber above a light box that can be converted to either white or ultraviolet light. Images of protein, DNA and RNA resolved in gels can be obtained after staining and appropriate illumination in the chamber. The high resolution and sensitivity of the camera combined with wavelength selective filters enable capture and accurate quantification of faint and bright signals and allowing us to undertake applications not previously possible with older imagers. The research being conducted by the five groups requesting this imager spans a range of basic science investigations including: determining the structure of individual cell membrane-spanning proteins, dissecting structure-function relationships of enzymes, determining how a parasitic DNA element replicating in a cell nucleus borrows host functions to ensure its own maintenance, understanding how packaging of our chromosomal DNA influences expression of our genes and comparing the effects of different fatty acids on lipid metabolism and their impact on atherosclerosis. The imager will also be accessible to research groups other than those named in this application thereby facilitating the research of many.