UV VIS microplate spectrophotometer for studies on the environmental physiology of aquatic animals

该提案要求为研究工具和仪器赠款提供资金，类别1，用于购买微孔板分光光度计。新设备将用于支持水生动物生理学和对金属等环境污染物的反应的研究计划。需要一种新的分光光度计来取代非常旧的手动比色皿式分光光度计。旧仪器不具备发展中的研究计划所需的能力。选择了能够阅读微孔板的分光光度计。与传统的比色皿型分光光度计相比，微孔板光度计在光学质量方面几乎相同。微孔板的能力提供了许多独特的优势，包括：每次检测的成本较低，废物和危险废物较少，更高的通量率，易于使用，调整检测参数的灵活性，以及更好的数据再现性。每次测定的成本较低是由于微孔板技术使用的体积小。小体积反过来又减少了产生的废物量。微量滴定仪的真实的好处之一是节省时间。过去在老式的温度计上需要数小时的测量现在可以在几分钟内完成。购买微孔板分光光度计将为实验室研究计划增加新的功能，并允许更多相关的测量。此外，它将鼓励更好的实验设计和更有效地利用资源，包括降低成本。

This proposal requests funding for a Research Tools and Instruments Grant, category 1, to purchase a microplate spectrophotometer. The new equipment would be used to support research programs on the physiology of aquatic animals and responses to environmental contaminants such as metals. A new spectrophotometer is needed to replace the very old manual cuvette style spectrophotometer. The old instrument does not have the capabilities required for the developing research program. A spectrophotometer that is capable of reading microplates has been chosen. Microplate spectrophotometers are virtually equal interms of optical quality compared to a traditional cuvette type of spectrophotometer. The microplate capabilities offer a number of distinct advantages including: lower cost per assay, less waste and less hazardouswaste, much higher throughput rates, ease of use, flexibility for adjusting assay parameters, and better data reproducibility. The lower costs per assay is due to the small volumes used with microplate technology. The small volumes in turn reduced the amount of waste produced. One of the real benefits of microplatespectrophotometers is the savings in time. Measurements that used to take hours on the older style spectrophotometers now can be done in a few minutes. The purchase of a microplate spectrophotometer will add new capabilities to the lab research program and allow more relevant measurements. As well, it will encourage better experimental design and more efficient use of resources including the lowering of costs.