Studies of proteins at interfaces and modification of surfaces for biotechnology applications

(1)蛋白质界面行为：蛋白质在界面上有很强的吸附倾向，这对生物技术的许多领域（如食品加工、诊断系统、生物传感器和蛋白质分离和纯化）都有重要的影响。有必要提高对蛋白质吸附的理解，以便根据需要对其进行控制。我们将解决以下广泛的问题：(a)蛋白质表面结合的性质，(b)吸附蛋白质的取向，(c)构象变化和生物功能的改变。我们将研究选定的蛋白质表面系统的吸附动力学和平衡，并建立吸附的机理模型。这些模型将包含特定的取向和随时间变化的构象变化。在这项工作中，我们使用了先进的实验方法，如中子反射法（与NRC Chalk River合作）和需要同步辐射的方法（加拿大光源）。

(1) Protein interfacial behaviour: Proteins have a strong tendency to adsorb at interfaces and this has important consequences for a number of areas of biotechnology (eg food processing, diagnostic systems, biosensors and protein separation and purification). There is a need for improved understanding of protein adsorption so that it can be controlled as required for a given purpose. We will be addressing the following broad questions: (a) the nature of protein surface binding, (b) the orientation of adsorbed proteins, (c) conformational changes and alteration of biologic function. We will study the adsorption kinetics and equilibria of selected protein-surface systems and develop mechanistic models of adsorption. The models will incorporate specific orientation and time dependent conformational changes. In this work we make use of advanced experimental methods such as neutron reflectometry (collaboration with NRC Chalk River) and methods requiring synchrotron radiation (Canadian Light Source).