Molecular mechanisms of fungal mycoparasitism

线虫寄生于马铃薯丝核菌病的病原菌立枯丝核菌（Rhizoctoniasolani）。它作为一种真菌寄生虫的成功很大程度上是由于它拥有一套降解R.索拉尼 这些酶被用作抗真菌化合物，当它们组合使用时，它们的有效性得到增强。该提案的目的是通过提供微生物-微生物相互作用期间发生的许多生物过程的全景，为更广泛的真菌寄生提供帮助。这将通过以下方式实现：（i）产生包含匿名cDNA克隆的消减EST文库;（ii）使用微阵列分析鉴定在真菌寄生虫和宿主的相互作用期间在它们两者中差异表达的基因;（iii）克隆作为最上调基因的基因;和（iv）研究这些基因在S. elegans的菌丝和菌核。使用定量实时逆转录（QRT）-PCR检测solani。

The fungus Stachybotrys elegans parasitizes the plant pathogen Rhizoctonia solani, the causal agent of Rhizoctonia disease of potato. Its success as a mycoparasite is due in large part to its possession of a suite of enzymes that degrade the cell walls of R. solani. These enzymes are used as anti-fungal compounds, and their effectiveness is enhanced when they are used in combination. The aim of this proposal is to contribute to the wider picture of mycoparasitism by providing a panoramic view of many biological processes occurring during microbe-microbe interaction. This will be accomplished by: (i) generating subtracted EST libraries comprising anonymous cDNA clones; (ii) identifying differentially expressed genes in both the mycoparasite and the host during their interaction using microarray analysis; (iii) cloning genes that are the most up-regulated genes and (iv) studying the differential and temporal expression of these genes during confrontation of S. elegans with hyphae and sclerotia of R. solani using quantitative real-time reverse-transcription (QRT)-PCR.