Single cell genetic profiling to reveal molecular and cellular changes in BRCA preneoplastic tissue

导致BRCA 1或BRCA 2突变女性患乳腺癌的初始分子和细胞事件尚不清楚。我们将使用最先进的基因组工具（单细胞RNA-seq和全基因组测序）来确定癌症是如何在缺乏正常BRCA基因的情况下开始的。来自癌前乳腺组织的干细胞和子细胞的单细胞基因组分析将用于识别可用于临床的早期指示分子变化。

The initial molecular and cellular events that lead to breast cancer in women with BRCA1 or BRCA2 mutations are unknown. We will use state-of-the-art genomic tools (Single Cell RNA-seq and whole genome sequencing) to determine how cancer begins in absence of normal BRCA genes. Single cell genomic profiling of stem and daughter cells from pre-cancerous breast tissue will be used to identify early-indicator molecular changes that could be exploited in the clinic.