Lantibiotics seperation and binding properties of metal-lantibiotics by capillary electrophoresis

本研究的目的是开发新的和有效的分析技术，用于分离，鉴定和定量细菌lantibiotic。另一个目的是研究金属与lantibiotic的关联，以及这些金属-lantibiotic络合物用于治疗和诊断目的的潜在用途。羊毛硫抗生素是由多种细菌产生的一组高度修饰的抗微生物肽。它们构成了一组新的抗生素，被发现可以抵御传统抗生素无效的传染病。羊毛硫抗生素已被用于治疗皮肤感染，用于对抗食品中的病原微生物，并且几种还具有抗肿瘤，抗癌和抗病毒活性。羊毛硫抗生素的分析对于优化发酵、产品分离、过程控制、确定其最佳剂量以及确定其在体液中的浓度至关重要。此外，羊毛硫抗生素的优化分离将有利于食品中药物残留的筛选。研究表明，在与金属螯合后，生物活性化合物在减缓或阻止癌症生长和阻碍细菌生长方面变得更加活跃。此外，它们的治疗潜力在与金属络合时增强。随着人们对羊毛硫抗生素作为经典抗生素的替代治疗剂的兴趣越来越大，重要的是研究金属与羊毛硫抗生素的结合如何可能扩大其治疗潜力，以及在病原体的诊断和治疗中的可能用途。为了获得这种理解，毛细管电泳将被用来调查的结合性能时，金属与lantibiotic以及金属lantibiotic复合物的结合到目标细菌。该分析将提供对lantibiotic结构、多样性和作用模式的全面了解，从而为制药、医疗和农业行业提供宝贵的工具。

The goal of this research is to develop novel and efficient analytical techniques for separating, identifying, and quantifying bacterial lantibiotics. The other objective is to investigate the association of metals with lantibiotics and the potential utilization of these metal-lantibiotic complexes for therapeutic and diagnostic purposes. Lantibiotics are a diverse group of heavily modified antimicrobial peptides produced by a wide range of bacteria. They constitute a new group of antibiotics that are being found to ward off infectious diseases against which classic antibiotics are ineffective. Lantibiotics have been used in treating skin infections, for combating pathogenic microorganisms in food, and several have also been characterized for their anti-tumour, anti-cancer and anti-viral activity. Analysis of lantibiotics will be essential for optimization of fermentation, product isolation, process control, determining their optimal dosages, and for determining their concentration in body fluids. Furthermore, an optimized separation of lantibiotics will be advantageous in the screening of food products for drug residues. Studies have indicated that upon chelation with metals, biologically-active compounds become more active in slowing or stopping the growth of cancer and in hampering the growth of bacteria. In addition, their therapeutic potential is enhanced on complexation with metals. With growing interest in lantibiotics as alternative therapeutic agents to classic antibiotics, it is important to investigate how the binding of metal to lantibiotics might expand their therapeutic potential, and possible use in the diagnosis and treatment of pathogenic organisms. To gain this understanding, capillary electrophoresis will be used to investigate the binding properties when metals associate with lantibiotics as well as binding of the metal-lantibiotic complex to target bacteria. This analysis will provide a comprehensive understanding of lantibiotic structure, diversity and modes of action, thus providing an invaluable tool to pharmaceutical, medical and agricultural industries.