Quantitative polymerase chain reaction equipment

该提案旨在申请设备补助金（RTI），以确保为定量聚合酶链反应系统提供资金，以支持卡尔加里大学生物科学系六个分子生物学实验室的研究。所有六个实验室都在研究微生物系统。购买的设备将用于多种应用，包括监测固氮根瘤、自由生活细菌细胞（野生型和调节突变体）和生物膜中细菌的基因表达。其他预期的用途包括估计野生型质粒和最小复制子的质粒拷贝数，以及间接定量环境和植物根瘤中的细菌。该设备将被所有共同申请者的实验室大量使用。

This proposal is for an equipment grant (RTI) to secure funding for a quantitative polymerase chain reaction system to support the research of six molecular biology laboratories in the Department of Biological Sciences at the University of Calgary. All six laboratories are working with microbial systems. The equipment purchased would be used for a variety of applications, including monitoring of gene expression in bacteria in nitrogen fixing root nodules, free-living bacterial cells (wild type and regulatory mutants) and biofilms. Other anticipated uses include estimating plasmid copy number of wild type plasmids and minimum replicons, and quantifying bacteria indirectly in the environment and in plant nodules. This equipment will be heavily used by all of the co-applicants' laboratories.