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STTR Phase I: COVID-19 - High-Brightness Fluorescent Probes for Quantitative Polymerase Chain Reaction

STTR 第一阶段：COVID-19 - 用于定量聚合酶链式反应的高亮度荧光探针

基本信息

批准号：
2034693
负责人：
Xiuling Liu
金额：
$ 25.6万
依托单位：
StabiLux Biosciences, Inc
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-15 至 2022-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2034693&HistoricalAwards=false
关键词：
STTR Phase COVID 19 Brightness

项目摘要

The broader impact/commercial potential of this Small Business Technology Transfer (STTR) Phase I project is to develop a new probe to detect low levels of viral load, such as that causing COVID-19. Rapid and reliable kits are not currently readily available. This project will provide rapid detection of viral material using exsiting cost-effective techniques with fewer detection errors. Being able to quantify and track viral loads at low concentration and high accuracy will expedite tests, reduce false negatives, and accelerate vaccine and drug development to combat COVID-19, and future pandemics.This STTR Phase I project will develop innovative fluorescent probes based on high-brightness fluorophores/dyes (HBFs) that offer tunable extinction coefficients up to several thousand times higher than commercial dyes. Based on this HBF platform technology, we will be able to create high-brightness qPCR probes that can specifically quantify viral RNAs by strong and tunable fluorescent signals at fewer amplification cycles and minimum replication errors. The activity, efficiency, and specificity of high-brightness qPCR probes will be evaluated in the project.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该小企业技术转让 (STTR) 第一阶段项目更广泛的影响/商业潜力是开发一种新的探针来检测低水平的病毒载量，例如导致 COVID-19 的病毒载量。目前还没有快速可靠的套件。该项目将使用现有的具有成本效益的技术来快速检测病毒材料，并减少检测错误。能够以低浓度和高精度量化和跟踪病毒载量将加快测试，减少假阴性，并加速疫苗和药物的开发，以对抗 COVID-19 和未来的大流行。该 STTR 第一阶段项目将开发基于高亮度荧光团/染料 (HBF) 的创新荧光探针，其可调消光系数比商业染料高出数千倍。基于这种 HBF 平台技术，我们将能够创建高亮度 qPCR 探针，通过强烈且可调节的荧光信号，以更少的扩增周期和最小的复制错误来特异性定量病毒 RNA。该项目将评估高亮度 qPCR 探针的活性、效率和特异性。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。