UV/visible spectrophotometer

紫外/可见分光光度计

• 批准号: 344942-2007
• 负责人: Hynes, Michael
• 金额: $ 1.36万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=326071
• 关键词: UV; visible; spectrophotometer

Research in my lab focuses on the molecular genetics of Rhizobium leguminosarum, a bacterium capable of forming nitrogen fixing nodules on the roots of several important crop plants.  Various projects in the lab are directed at understanding the role of genome organisation (particularly the large number of plasmids) in the biology of Rhizobium, and at identifying factors which contribute to the ecological success of individual strains of this bacterium.  Many of the experiments require measurements of gene expression using gene fusions to the lacZ and gusA genes, and various other enzyme assays are also requried.  This application is a request for funding for a spectrophotometer to use in enzyme assays and other molecular biological protocols.  This instrument would be shared with Dr. H. Ceri's lab, which is also involved in molecular studies of bacteria, particularly with regard to their ability to form biofilms. Studies in the Ceri lab focus on gene expression in biofilms and the role of biofilms in infections and in resistance to antibiotics.

我实验室的研究主要集中在根瘤菌的分子遗传学上，根瘤菌是一种能够在几种重要作物的根部形成固氮根瘤的细菌。实验室的各种项目都旨在了解基因组组织的作用（特别是大量的质粒）在根瘤菌生物学中，许多实验需要使用与lacZ和gusA基因的基因融合来测量基因表达，以及其他各种酶分析也是需要的。这个申请是一个分光光度计的资金申请，用于酶分析和其他分子生物学协议。这个仪器将与H. Ceri的实验室也参与了细菌的分子研究，特别是关于它们形成生物膜的能力。Ceri实验室的研究重点是生物膜中的基因表达以及生物膜在感染和抗生素耐药性中的作用。