UV/visible spectrophotometer

我实验室的研究重点是豆科根瘤菌的分子遗传学，这种细菌能够在几种重要作物的根上形成固氮结节。实验室的各种项目旨在了解根瘤菌生物学中基因组组织（特别是大量质粒）的作用，并确定有助于这种细菌个别菌株生态成功的因素。许多实验需要使用lacZ和gusA基因的基因融合来测量基因表达，还需要各种其他酶测定。本申请是为一种用于酶分析和其他分子生物学协议的分光光度计申请资金。这台仪器将与H. Ceri博士的实验室共享，该实验室也参与细菌的分子研究，特别是关于它们形成生物膜的能力。Ceri实验室的研究重点是生物膜中的基因表达以及生物膜在感染和抗生素耐药性中的作用。

Research in my lab focuses on the molecular genetics of Rhizobium leguminosarum, a bacterium capable of forming nitrogen fixing nodules on the roots of several important crop plants.  Various projects in the lab are directed at understanding the role of genome organisation (particularly the large number of plasmids) in the biology of Rhizobium, and at identifying factors which contribute to the ecological success of individual strains of this bacterium.  Many of the experiments require measurements of gene expression using gene fusions to the lacZ and gusA genes, and various other enzyme assays are also requried.  This application is a request for funding for a spectrophotometer to use in enzyme assays and other molecular biological protocols.  This instrument would be shared with Dr. H. Ceri's lab, which is also involved in molecular studies of bacteria, particularly with regard to their ability to form biofilms. Studies in the Ceri lab focus on gene expression in biofilms and the role of biofilms in infections and in resistance to antibiotics.