Checkpoint protein bioassays for detecting genotoxicants in water

准确监测水质对于保护水生生态系统至关重要。随着人类活动的加强，如制造业、农业和市政当局的发展，加拿大河流和湖泊致癌物质污染水平增加的风险正在上升。我们建议开发一种快速检测方法，通过使用识别检查点蛋白的抗体来筛选水样。检查点蛋白作为一种防御机制来保持基因组的完整性，当DNA损伤发生时，它们的水平会迅速上升。通过将鱼类细胞暴露在潜在污染地点的水中，我们可以监测由于此类致癌物的存在而导致检查点蛋白水平上升的程度。这项技术将允许对水样进行高度敏感和有效的筛选，并将极大地帮助确定最脆弱的水生生态系统以及保护它们的最佳措施。

Accurate monitoring of water quality is of primary importance in protecting aquatic ecosystems. With the intensification human activities, such as manufacturing, agriculture, and the growth of municipalities, the risk of increased levels of carcinogen contamination in Canadian rivers and lakes is rising. We propose to develop a rapid test to screen water samples by using antibodies that recognize checkpoint proteins. Checkpoint proteins act as a defense mechanism to preserve genome integrity, and their levels are known to increase quickly when DNA damage occurs. By exposing fish cells to water from potentially contaminated sites, we can monitor the extent to which checkpoint protein levels rise as a consequence of the presence of such carcinogens. This technology will allow for highly sensitive and efficient screening of water samples, and will greatly aid in identifying the most vulnerable aquatic ecosystems, and the best measures to protect them.