Lantibiotics seperation and binding properties of metal-lantibiotics by capillary electrophoresis

本研究的目的是开发新的、有效的分离、鉴定和定量细菌抗生素的分析技术。另一个目的是研究金属与抗生素的关系，以及这些金属-抗生素复合物在治疗和诊断方面的潜在应用。抗生素是一种由多种细菌产生的经过大量修饰的抗菌肽。它们构成了一组新的抗生素，被发现可以抵御传统抗生素无效的传染病。抗生素已被用于治疗皮肤感染、对抗食品中的致病微生物，其中一些抗生素还具有抗肿瘤、抗癌和抗病毒活性。抗生素的分析对于优化发酵、产品分离、过程控制、确定其最佳剂量以及确定其在体液中的浓度至关重要。此外，对l抗生素的优化分离将有利于食品中药物残留的筛选。研究表明，在与金属螯合后，生物活性化合物在减缓或阻止癌症的生长和阻碍细菌的生长方面变得更加活跃。此外，它们的治疗潜力与金属络合增强。随着人们对l抗生素作为经典抗生素的替代治疗药物的兴趣日益浓厚，研究金属与l抗生素的结合如何扩大其治疗潜力，以及在病原生物的诊断和治疗中可能的应用是很重要的。为了了解这一点，毛细管电泳将用于研究金属与抗生素结合时的结合特性以及金属-抗生素复合物与目标细菌的结合。这一分析将提供对抗生素结构、多样性和作用方式的全面了解，从而为制药、医疗和农业行业提供宝贵的工具。

The goal of this research is to develop novel and efficient analytical techniques for separating, identifying, and quantifying bacterial lantibiotics. The other objective is to investigate the association of metals with lantibiotics and the potential utilization of these metal-lantibiotic complexes for therapeutic and diagnostic purposes. Lantibiotics are a diverse group of heavily modified antimicrobial peptides produced by a wide range of bacteria. They constitute a new group of antibiotics that are being found to ward off infectious diseases against which classic antibiotics are ineffective. Lantibiotics have been used in treating skin infections, for combating pathogenic microorganisms in food, and several have also been characterized for their anti-tumour, anti-cancer and anti-viral activity. Analysis of lantibiotics will be essential for optimization of fermentation, product isolation, process control, determining their optimal dosages, and for determining their concentration in body fluids. Furthermore, an optimized separation of lantibiotics will be advantageous in the screening of food products for drug residues. Studies have indicated that upon chelation with metals, biologically-active compounds become more active in slowing or stopping the growth of cancer and in hampering the growth of bacteria. In addition, their therapeutic potential is enhanced on complexation with metals. With growing interest in lantibiotics as alternative therapeutic agents to classic antibiotics, it is important to investigate how the binding of metal to lantibiotics might expand their therapeutic potential, and possible use in the diagnosis and treatment of pathogenic organisms. To gain this understanding, capillary electrophoresis will be used to investigate the binding properties when metals associate with lantibiotics as well as binding of the metal-lantibiotic complex to target bacteria. This analysis will provide a comprehensive understanding of lantibiotic structure, diversity and modes of action, thus providing an invaluable tool to pharmaceutical, medical and agricultural industries.