Posttranslational modification-specific proteomics approach to identification of molecular biomarkers for wheat seed dormancy control and regulation

穗发芽(PHS)是小麦生产的永久威胁。小麦发芽可能导致生产者在价格/吨上遭受高达40%的巨大收成损失，因为发芽的谷物是一个重要的降级因素。种子休眠是由遗传和环境因素控制的，它应该足够强，以抑制萌发。PHS抗性是一种等级保护形式，最好地通过遗传抗性构建在小麦品种中。然而，对于休眠控制过程中发生的过程和分子信号机制仍然知之甚少。该研究计划旨在研究小麦种子休眠和发芽抗性的来源。提出了休眠的蛋白质组学分析的方方面面--连接基因组和蛋白质组的生物标志物模式，控制特定表型表达可塑性的遗传因素，翻译后信号传递，植物激素介导的反应和环境变化的影响。在接下来的五年里，对差异蛋白质相关图谱的深入系统分析，包括定位、动力学和相互作用伙伴，将集中在特定的和功能相关的‘亚蛋白质组’上。分离抗休眠/发芽的加倍单倍体群体的杂交系将被用来验证所鉴定的候选生物标记蛋白。这项研究将为小麦抗萌发和休眠控制的生化性质创造新的知识，将有助于理解在不利环境条件下减少小麦种子休眠的生化性质，并为持续的、具有国际竞争力的品种开发确定分子基础和遗传资源。本研究的结果是通过对休眠生物标志物的系统蛋白质组学分析，对种质资源的抗发芽能力进行了全面的评价。来自加拿大精英遗传学的种质的广泛基础确保了长期选择和近期品种开发的遗传多样性。

Pre-harvest sprouting (PHS) is a perpetual threat to wheat production. Sprouting in wheat can result in massive harvest losses of up to 40% in the price/tonne for producers because sprouted grain is an important downgrading factor. Seed dormancy, which is controlled by genetic and environmental factors, should be strong enough to inhibit germination. PHS resistance is a form of grade protection that is best built into wheat cultivars through genetic resistance. However, still very little is known about the processes and molecular signalling mechanisms occurring during dormancy control. The research program aims to examine sources of wheat seed dormancy and sprouting resistance. I propose the proteomic analysis of dormancy in all aspects - biomarker pattern linking genome to proteome, genetics factors control to specific phenotypic expression plasticity, post-translational signalling, phytohormone-mediated response and effect of environmental changes. In the next five years, in-depth systematic analysis of differential protein correlation profiling including localization, dynamics, and interaction partners will be focused on specific and functionally relevant 'sub-proteomes'. The hybrid lines of doubled haploid populations segregating for dormancy/sprouting resistance will be used to validate the identified candidate biomarker proteins. The research will create new knowledge regarding the biochemical nature of sprouting resistance and dormancy control, will lead to understanding of the biochemical nature for reduced wheat seed dormancy under unfavourable environmental conditions and identify molecular basis and genetic resources for sustained, internationally competitive, variety development. The outcome of this research is the thorough evaluation of germplasm enhanced for sprouting resistance by the systematic proteomic analysis of dormancy biomarkers. The broad basis of the germplasm, derived from elite Canadian genetics, ensures genetic diversity for long term selection as well as for immediate variety development.