Fast protein liquid chromatography (FPLC) system for the structural studies of the PHD finger proteins in yeast

申请购买快速蛋白液相色谱（FPLC）蛋白纯化系统的资金，以帮助由约克大学新研究员Yi Sheng博士领导的酵母植物同源结构域（PHD）手指蛋白的结构和功能研究。PHD指蛋白是一种小的锌结合蛋白，被发现参与许多重要的细胞过程，如基因调控、免疫和发育。所要求的设备将用于纯化酵母PHD手指蛋白，用于三维结构测定和与其他细胞蛋白的相互作用研究。本研究的结果将有助于深入了解PHD指蛋白的功能差异，并揭示这些PHD指的突变如何影响细胞过程。FPLC系统是本研究成功的关键。

Funds to purchase a Fast Protein Liquid Chromatography (FPLC) protein purification system are requested to aid the structural and functional studies of the yeast Plant Homeo-Domain (PHD) finger proteins led by the new investigator Dr. Yi Sheng at York University. The PHD finger proteins are small zinc-binding proteins, found to be involved in many important cellular processes such as gene regulation, immunity and development. The requested equipment will be used to purify yeast PHD finger proteins for three-dimensional structure determination and interaction studies with other cellular proteins. The outcome of this research will provide insight into the functional divergence of PHD finger proteins and reveal how mutations in these PHD finger affect cellular processes. The FPLC system is essential to the success of the proposed research.