Lantibiotics seperation and binding properties of metal-lantibiotics by capillary electrophoresis

这项研究的目标是开发新的和有效的分析技术来分离、鉴定和定量细菌抗生素。另一个目的是研究金属与抗生素的关系，以及这些金属-抗生素络合物在治疗和诊断方面的潜在用途。抗生素是由多种细菌产生的一组高度修饰的抗菌肽。它们构成了一组新的抗生素，正在被发现可以预防传统抗生素无效的传染病。抗生素已被用于治疗皮肤感染，对抗食品中的致病微生物，还有几种抗生素也因其抗肿瘤、抗癌和抗病毒活性而被鉴定。抗生素的分析对于优化发酵、产品分离、过程控制、确定它们的最佳剂量以及确定它们在体液中的浓度是必不可少的。此外，抗生素的优化分离将有利于食品中药物残留的筛选。研究表明，在与金属螯合后，生物活性化合物在减缓或阻止癌症生长以及阻碍细菌生长方面变得更加活跃。此外，它们与金属络合后的治疗潜力也得到了增强。随着人们对抗生素作为传统抗生素的替代治疗药物的兴趣与日俱增，研究金属与抗生素的结合如何扩大它们的治疗潜力以及在病原体诊断和治疗中的可能用途是很重要的。为了了解这一点，将使用毛细管电泳法研究金属与抗生素结合时的结合特性以及金属-抗生素络合物与靶细菌的结合。这一分析将使人们全面了解抗生素的结构、多样性和作用方式，从而为制药、医疗和农业提供宝贵的工具。

The goal of this research is to develop novel and efficient analytical techniques for separating, identifying, and quantifying bacterial lantibiotics. The other objective is to investigate the association of metals with lantibiotics and the potential utilization of these metal-lantibiotic complexes for therapeutic and diagnostic purposes. Lantibiotics are a diverse group of heavily modified antimicrobial peptides produced by a wide range of bacteria. They constitute a new group of antibiotics that are being found to ward off infectious diseases against which classic antibiotics are ineffective. Lantibiotics have been used in treating skin infections, for combating pathogenic microorganisms in food, and several have also been characterized for their anti-tumour, anti-cancer and anti-viral activity. Analysis of lantibiotics will be essential for optimization of fermentation, product isolation, process control, determining their optimal dosages, and for determining their concentration in body fluids. Furthermore, an optimized separation of lantibiotics will be advantageous in the screening of food products for drug residues. Studies have indicated that upon chelation with metals, biologically-active compounds become more active in slowing or stopping the growth of cancer and in hampering the growth of bacteria. In addition, their therapeutic potential is enhanced on complexation with metals. With growing interest in lantibiotics as alternative therapeutic agents to classic antibiotics, it is important to investigate how the binding of metal to lantibiotics might expand their therapeutic potential, and possible use in the diagnosis and treatment of pathogenic organisms. To gain this understanding, capillary electrophoresis will be used to investigate the binding properties when metals associate with lantibiotics as well as binding of the metal-lantibiotic complex to target bacteria. This analysis will provide a comprehensive understanding of lantibiotic structure, diversity and modes of action, thus providing an invaluable tool to pharmaceutical, medical and agricultural industries.