Biochemical, Genomic and Phenomic Analysis of Gastric Parietal Cells from Wildtype and Mutant Mice

野生型和突变小鼠胃壁细胞的生化、基因组和表型分析细胞和环境之间的界面是细胞膜。信号、营养物质和离子都必须穿过这道屏障。此外，这些细胞含有许多特化的细胞内膜室。我们对指导这些结构的合成并决定其最终组成和形状的信号知之甚少。这项资助将利用胃中的酸性分泌细胞来研究这些问题，因为它们包含一个非常广泛的膜系统。我们将使用最先进的遗传和细胞生物技术在整个动物环境中操纵和分析这些细胞。

Biochemical, Genomic and Phenomic Analysis of Gastric Parietal Cells from Wildtype and Mutant Mice. The interface between the cell and its environment is the cell membrane. Signals, nutrients, and ions all have to cross this barrier. In addition, the cells contain many specialized intracellular membranous compartments. We know little about the signals that direct the synthesis of these structures and determine their final composition and shape. This grant will utilize acid secretory cells in the stomach to examine these questions because they contain a very extensive membrane system. We will use a state-of-the-art genetic and cell biological technologies to manipulate and analyse these cells in a whole animal setting.