GROWTH AND OPTIMIZATION OF AUTOTROPHIC-TO-HETEROTROPHIC MICROALGAE FOR LIPID PRODUCTION USING LOW COST CARBON SOURCES

加拿大嘉实生物能源的综合醋酸盐生物燃料精炼工艺利用生物质废弃物(如粪便)、电力、阳光和有机碳提供可再生能源，并将其输送到封闭式生物反应器，用于生产藻类生物柴油。由于藻类生长速度快，油脂浓度高，在同等面积的土地上生产的生物燃料是传统油籽的20多倍，使其成为一种有吸引力的低成本非食品原料。最大的挑战是优化流程，使其有利可图、具有竞争力。加拿大丰实公司将高产高效的推流光生物反应器与现有的石油和天然气水处理技术相结合，以减少资本和运营费用。与竞争对手不同的是，CanHarest使用低密度的生长过程，使用连续的光折光槽，然后使用稀醋酸进行黑暗的生长，从而保持低运营成本和高效率。这些细胞在巨大的黑暗储罐中缺乏氮气和光线，并在不需要额外催化剂和昂贵的气液转化过程的情况下生产低级醋酸来产生脂质。加拿大丰收公司正在与奥兹学院创新学院建立新的合作伙伴关系，以进行应用研究，以确认在这种独特的生长模式下，使用两种藻类品种的藻类生长产量。需要确认预期产量，以确保CanHarest全面商业炼油厂的可行性，并确保公司业务计划的可行性。藻类生物燃料产业将产生显著的环境效益，包括二氧化碳封存、废水营养循环和减少化石燃料的使用。这一新兴产业在中国的发展

CanHarvest Bioenergy's Integrated Acetate Biofuel Refinery process provides a renewable energy source using biomass waste (such as manure), power, sunlight and organic carbon fed to closed bioreactors for the production of algae biodiesel. Algae produce over twenty times the amount of biofuel than conventional oilseeds on an equal amount of land due to rapid growth rates and high concentrations of lipids, making it an attractive low-cost non-food feedstock. The largest challenge is optimizing the process so that it is profitable and competitive. CanHarvest incorporates high yield high efficiency plug-flow photobioreactors with existing oil and gas water treatment technology to reduce capital and operating expenses. Unlike the competitors, CanHarvest uses growth processes at low densities with continuous photobaffle tanks followed by a dark period of growth using dilute acetic acid, keeping operating costs low and efficiencies high. The cells are starved of nitrogen and light in large dark tanks and low grade acetic acid to produce lipids without the need for additional catalysts and expensive gas-to-liquid processes. CanHarvest is forming a new partnership with Olds College School of Innovation to conduct applied research to confirm the algae growth yields using two algae varieties under this unique growth model. Confirmation of the expected yields is required to ensure the feasibility of a full scale commercial refinery for CanHarvest and ensure the viability of the company business plan. The algae biofuels industry will have significant environmental benefits including CO2 sequestration, wastewater nutrient recycling and reduction of the use of fossil fuels. Development of this new industry in