High performance centrifuges with rotors

这项申请要求为3台用于制备生物样品的高性能离心机和处理大量样品所需的配套转子提供资金。典型的用途包括从培养物中收集细胞、分级细胞裂解物、纯化DNA和蛋白质、有机混合物的相分离和收集纳米颗粒。一台离心机将位于生物危害防护2级专用房间，一台离心机将位于生物服务设施（一个提供全部门使用生物相关设备的设施），一台离心机将位于Vederas实验室。 所要求的转子和适配器可以处理几毫升到几升大小的样品。属于15个研究小组的大约80名成员每天多次使用这些离心机。 由于大量用户分布在一座两翼、5层楼的建筑物内，而且需要专门为生物危害控制2级工作配备一台离心机，因此必须至少配备3台离心机。

This application requests funding for 3 high performance centrifuges for the preparation of biological samples and the accompanying rotors needed to process a wide range of sample volumes. Typical uses include collecting cells from culture, fractionating cell lysates, purification of DNA and proteins, phase separation of organic mixtures and collection of nano-particles. One centrifuge will be located in a biohazard containment level 2 dedicated room, one centrifuge will be located in the Biological Services facility (a facility providing department-wide access to biology-related equipment), and one centrifuge will be located in the Vederas lab. The rotors and adaptors requested allow for processing of samples several millilitres to litres in size. These centrifuges are used many times per day by approximately 80 members belonging to 15 research groups. It is essential that at least 3 centrifuges be available due to the high number of users spread in location over a 2-wing, 5-story building and also the need to dedicate one centrifuge for biohazard containment level 2 work.