Fast Real Time Quantitative Polymerase Chain Reaction Thermocycler

我的研究计划专注于阐明甲状腺激素(TH)作用于细胞的基本机制，并开发分子工具来检测破坏TH作用的环境污染物。此外，我们在设计非致命性和微创组织采样技术方面的专业知识与mRNA转录水平评估相结合，使我们能够为创建针对各种不同动物物种的野生动物健康评估的创新方法做出重大贡献。我们工作的关键是对mRNA转录水平的分析，我们完全依赖于实时定量聚合酶链式反应(QPCR)。该实验室制作的每一份手稿都包含了QPCR。我们正处于灾难的边缘。我们实验室中仅有的两台老化的Stratagene Real Time-QPCR热循环仪最近发生了多起同时故障，严重影响了多个研究项目。我们非常需要更换这些设备，并要求Eppendorf Realplex 4S系统能够同时更换这两种设备。所请求的配置具有更坚固的设计，非常适合高使用量和多用户。银块提供了可用的最快斜坡时间，从而将运行时间减少到不到竞争对手系统的一半。随附的温度梯度功能将有助于底漆优化，进一步简化机器的使用。

My research program focuses on elucidating the fundamental mechanisms whereby thyroid hormones (THs) act on cells and developing molecular tools to detect environmental contaminants that act to disrupt TH action. Moreover, our expertise in devising non-lethal and minimally invasive tissue sampling techniques combined with mRNA transcript level assessment has allowed us to make significant contributions to creating innovative ways for assessing wildlife health on a wide range of diverse animal species. The linchpin in our work is the analysis of mRNA transcript levels and we are absolutely dependent upon quantitative real-time polymerase chain reaction (QPCR). Every manuscript produced by the lab has included QPCR. We are at the brink of disaster. The only two aging Stratagene real time-QPCR thermocyclers that we have in the lab have recently suffered multiple simultaneous breakdowns that severely impacted multiple research projects. We are in great need of a replacement for these units and are requesting an Eppendorf Realplex 4S system that is capable of replacing both. The requested configuration has a more robust design ideal for heavy use and multiple users. The silver block provides the fastest ramp times available thereby reducing run times to less than half of competing systems. The accompanying temperature gradient feature will facilitate primer optimization further streamlining use of the machine.