Flow cytometer for cellular analysis

需要一台流式细胞仪来进行生物化学、细胞生物学、环境毒理学、发育和病毒学六个nserc资助的研究项目所需的细胞分析，旨在了解：(1)应激（氧化应激、农药）诱导的适应性细胞反应和凋亡的机制；(2)有毒金属致细胞功能障碍的细胞信号、适应性反应和氧化应激机制；(3)环境污染物（农业农药、化肥）损害哺乳动物细胞代谢功能的机制；(4)神经嵴细胞中表达的对肠道神经系统正常形成至关重要的新基因的功能；(5)抗氧化剂铜蓝蛋白在神经系统发育中的作用；(6)人内源性逆转录病毒包膜基因在人滋养细胞中的表达调控。该设备是由3名高级研究员（D. Averill-Bates， C. Jumarie， J. Paquin）和3名年轻研究人员（B. Barbeau， N. Pilon， M. Boily）申请的。流式细胞仪可以快速分析单个细胞中的多种生物学参数。用荧光探针标记的细胞悬浮液通过聚焦光束，对单个细胞的敏感测量被检测并可以量化。要求的C6 Accuri流式细胞仪配备一个蓝色和红色激光器，两个光散射检测器和四个荧光检测器（FL1-FL4）。使用2激光激发是高度通用的，并允许使用更广泛的荧光染料。4个检测器能够使用4种不同的荧光染料同时分析同一细胞样品中的4个参数。流式细胞仪将用于分析各种细胞参数：活性氧；细胞凋亡和自噬检测；细胞活力、增殖和分化；蛋白质磷酸化;细胞表面蛋白和受体的表达；还有基因表达。流式细胞仪是高质量的，有竞争力的研究，在加拿大和国际水平，和研究生培训的实验室，这6名nserc资助的研究人员在生物和化学系必不可少的。

A Flow Cytometer is requested for the cellular analyses required for six NSERC-funded research programs in Biochemistry, Cell Biology, Environmental Toxicology, Development, and Virology, which aim to understand the: (1) Mechanisms underlying adaptive cellular responses and apoptosis induced by stresses (oxidative stress, pesticides); (2) Mechanisms involving cell signaling, adaptive responses and oxidative stress in cellular dysfunction caused by toxic metals; (3) Mechanisms by which environmental pollutants (agricultural pesticides, fertilisers) impair metabolic function in mammalian cells; (4) Function of new genes expressed in neural crest cells that are critical for proper formation of the enteric nervous system; (5) Function of the antioxidant ceruloplasmin in nervous system development; and (6) Regulation of the expression of Human Endogenous Retrovirus envelope genes in human trophoblasts. The equipment is requested by 3 senior investigators (D. Averill-Bates, C. Jumarie, J. Paquin) and 3 young researchers (B. Barbeau, N. Pilon, M. Boily). A flow cytometer allows rapid analyses of a multitude of biological parameters in individual cells. Suspensions of cells labeled with fluorescent probes flow through a focused light beam and sensitive measurements on single cells are detected and can be quantified. The requested C6 Accuri Flow cytometer is equipped with a blue and red laser, two light scatter detectors, and four fluorescence detectors (FL1-FL4). The use of 2 lasers for excitation is highly versatile and allows use of a wider range of fluorichromes. The 4 detectors enable the simultaneous analysis of 4 parameters in the same cellular sample using 4 different fluorichromes. The flow cytometer will be used for analyses of a variety of cellular parameters: reactive oxygen species; apoptosis and autophagy assays; cell viability, proliferation and differentiation; protein phosphorylation; cell surface expression of proteins and receptors; and gene expression. The Flow Cytometer is essential for high quality, competitive research, at the Canadian and international levels, and graduate student training in the laboratories of these 6 NSERC-funded investigators in the Biology and Chemistry Departments.