Cell lysis apparatus and multiplex array reader

这笔赠款将购买一台细胞裂解装置，用于在不破坏细胞内容物的情况下打开各种细胞类型，以及一台进行各种分子生物分析的多路阵列读取器。这些设备是合理的配对，因为使用多路阵列读取器进行下游细胞和分子生物分析需要高质量的细胞裂解。所要求的设备对三所当地大学正在进行的研究项目至关重要，并将作为一种手段，在细胞、生理和整个生物体水平上整合分子生物学、生物信息学和生化数据。这些系统将成为威尔弗里德·劳里埃大学(WLU)公共设备室的一部分，并将由WLU、滑铁卢大学(UW)和圭尔夫大学(UG)的研究人员使用。该细胞裂解仪将取代老化的法国压力机，以提高从细菌、真菌、植物和动物细胞中提取的裂解物的质量。WLU、UW或UG目前未提供多路复用型阵列读取器。这项尖端技术将使生物分子相互作用(即。蛋白质-蛋白质和核酸-蛋白质)需要快速和有效地研究。这些共享的、可访问的研究设施仪器将有助于在申请者的实验室中培训HQP，以及综合生物学研究生项目学员。这些设备还将有助于发展新老调查人员之间的合作，并促进各机构之间对HQP的交叉培训。申请者的研究使用各种系统(例如病毒、鱼类、植物、真菌和细菌)来解决关于免疫、环境应激、疾病、分子相互作用、病原体检测以及蛋白质结构和功能的研究问题。因此，所要求的设备将帮助科学家探索跨机构的多学科研究问题。

This grant will purchase a cell lysis apparatus that will be used to break open a wide range of cell types without damaging their contents, and a multiplex array reader that conducts a broad variety of molecular bioassays. These pieces of equipment are a logical pairing, as quality lysis of cells is required for downstream cellular and molecular bioassays using the multiplex array reader. The equipment requested is vital to research programs being conducted at three local universities and will serve as a means of integrating molecular biology, bioinformatics, and biochemical data at the cellular, physiological, and whole organism levels. The systems will be part of a common equipment room at Wilfrid Laurier University (WLU) and will be used by researchers at WLU, the University of Waterloo (UW), and the University of Guelph (UG). The cell lysis apparatus will replace an aging French Press to improve lysate quality derived from bacterial, fungal, plant, and animal cells. A multiplex array reader is not currently available at WLU, UW or UG. This cutting edge technology will enable bio-molecular interactions (ie. protein-protein and nucleic acid-protein) to be studied quickly and efficiently. These shared, accessible, research facility instruments will contribute to the training of HQP in the applicants' laboratories, as well as Integrative Biology graduate program trainees. The equipment will also contribute to developing collaborations between new and established investigators and facilitate cross-training of HQP between institutions. Research by the applicants uses a variety of systems (e.g. viruses, fish, plants, fungi, and bacteria) to address research questions about immunity, environmental stress, disease, molecular interactions, pathogen detection, and protein structure and function. Therefore, the equipment requested will help scientists explore multi-disciplinary research questions across institutions.