COUPLING METABOLOMIC AND PROTEOMIC APPROACHES IN PROFILING ENDOBIOTICS METABOLISM

内生素是在体内形成的小分子，在发育和成人生理中发挥重要的生物学作用。这种作用的维持是由适当水平的合成和新陈代谢保证的。然而，代谢在内生菌活性控制中的确切作用仍不清楚，因为这一领域的研究人员面临着一个重大限制：即缺乏准确的方法来选择性和灵敏地测量代谢酶。在过去的5年里，新的最先进的技术，如LC-MS/MS，促进了新的科学学科的出现，这些学科被称为蛋白质组和代谢组学。这些学科的很大一部分致力于蛋白质和内生菌的具体鉴定。这一新的研究计划的目的是将这些新的学科联系起来，以产生一系列量化工具(称为切除前/代谢组对)，这些工具将有助于研究遗传和环境因素如何改变具有生物活性的内源物质的代谢和功能。事实上，这些工具将首次提供破译每种代谢酶在细胞对遗传或环境挑战的反应中的确切贡献的可能性。

Endobiotics are small molecules formed in the body that exert essential biological roles in development and adult physiology. The maintenance of such roles is ensured by adequate levels of synthesis and metabolism. However, the exact contribution of metabolism in the control of endobiotics activities is still unclear, since the researchers working in this field face a major limitation: i.e. the absence of accurate methods for the selective and sensitive measurement of metabolic enzymes. In the last 5 years, novel state of-the-art technologies, such as LC-MS/MS, have favoured the emergence of novel scientific disciplines named proteomic and metabolomic. A significant part of these disciplines is dedicated to the specific identification of proteins and endobiotics. The purpose of this novel research program is to associate these new disciplines in order to generate a series of quantification tools (called preotomic/metabolomic pairs) that will be useful in investigating how genetic and environmental factors can alter the metabolism and function of biologically active endogenous substances. Indeed, these tools will provide for the first time the possibility of deciphering the exact contribution of each metabolic enzyme in the cellular response to genetic or environmental challenges.