Effect of metallic nanoparticles on functional and structural properties of the photosynthetic apparatus

光系统II结构和电子传递活性的调节受到环境因素的强烈影响，例如引起抑制效应的不同污染物的存在。在这个研究项目中，我们试图了解光合器官的结构和功能是如何科普这些环境条件的。光合作用过程对环境胁迫的高度敏感性可用于开发生物分析工具，以评估环境中污染物的毒理学风险。由于纳米技术的发展和纳米材料在环境中的存在，我们开始研究不同类型的纳米颗粒对光合生物的影响，我们的假设是，在这些条件下，能量耗散机制将反映纳米颗粒诱导的抑制机制的特异性。我们的研究目标是研究抑制剂、纳米粒子在强光下的诱导效应和能量耗散机制之间的关系。通过使用高度先进的光谱研究和生物化学方法，将在体外和体内条件下进行实验。所获得的结果将提供可以解释植物如何在不同的光条件下存活和耐受纳米颗粒毒性效应的信息。这些结果将有助于一些纳米粒子的抑制作用和光合电子传递的必要性之间的相互作用的基础知识，并评估的可靠性的毒性生物标志物，以确定纳米粒子诱导的影响。

The regulation of photosystem II structure and electron transport activity is strongly affected by environmental factors such as the presence of different pollutants which cause inhibitory effects. In this research program, we attempt to understand in which way structure and functions of photosnthetic apparatus are accommodated to cope with those environmental conditions. The high sensitivity of photosynthetic processes towards environmental stress can be used for the development of bioanalytical tools to evaluate the toxicological risk of contaminants in the environment. Due to the recent development of nanotechnology and the possibility of nanomaterials being found in the environment, an evaluation of the possible effects of different types of nanoparticles on photosynthetic organisms was initiated.Our hypothesis is that, under those conditions, the mechanism of energy dissipation will reflect the specificity of the inhibition mechanism induced by nanoparticles. Our research objective is to study the relationships between the effects of inhibitors, nanoparticles induced under strong light intensity and energy dissipation mechanisms. By using highly advanced spectroscopic studies and biochemical methods, experiments will be done in vitro and in vivo conditions. Results obtained will provide information which can explain how plants can survive and tolerate nanoparticles toxicity effects under different light conditions. These results will contribute to fundamental knowledge concerning the interaction between inhibitory effects of some nanoparticles and on photosynthetic electron transport the necessity and to evaluate the reliability of toxicity biomarkers to identify nanoparticles-induced effects.