Effect of redox potential on the gene expression of Saccharomyces cerevisiae during very-high-gravity ethanol fermentation

本课题的目的之一是研究氧化还原电位对超高压乙醇发酵过程中酿酒酵母基因表达的影响。为了实现上述目标，将开发一种连续氧化还原电位控制的发酵工艺，使VHG乙醇发酵可以在指定的氧化还原电位水平下进行。一旦稳定状态建立，就收获酵母细胞，提取rna。然后应用微阵列技术获得特定氧化还原电位水平下的全基因表达谱。结果表明，基因表达模式与氧化还原电位之间存在相关性。研究人员将鉴定氧化还原电位调控的基因，并阐明它们在VHG条件下与乙醇生产有关的代谢功能。

One of the objectives of the proposal is to study the effect of redox potential on the gene expression of Saccharomyces cerevisiae during very-high-gravity (VHG) ethanol fermentation. To achieve the stated objective, a continuous redox potential-controlled fermentation process will be developed, so that VHG ethanol fermentation can be operated at a specified redox potential level. Once steady state is established, yeast cells are harvested and RNAs are extracted. Microarray technology is then applied to obtain whole gene expression profile under a specified redox potential level. As a result, correlation between gene expression pattern and redox potential is established. The genes modulated by redox potential will be identified and their metabolic functions relating to ethanol production under VHG conditions will be elucidated.