Cryogenic freezer chest for sample storage

所要求的低温冷冻箱可以在-150摄氏度下存储生物样本。在这种温度下保存样品不需要液氮。在-150℃时，样品内外不会发生冰重结晶，从而阻止样品的生物活性。随后可以对样品进行分析或重新分析，以验证数据的重复性。所要求的设备是一种通用工具，可以在很长一段时间内储存生物样本。从燃料酒精生产过程中收获的酵母样品将储存在所要求的冷冻箱中。这些样本将被用来进行全细胞基因表达和蛋白质表达谱，试图探索酵母在双重渗透(由于高糖浓度)和抑制(由于高乙醇水平)作用下的生理变化。因此，可以开发一种控制策略来减轻这种不利影响，从而为燃料酒精工业设计高产率的乙醇工艺。

The requested cryogenic freezer chest can store biological samples at -150 oC. No liquid nitrogen is required to keep stored samples at this temperature. At -150 oC, no ice re-crystallization will occur from within and outside samples and the biological activities of the sample are thus arrested. Samples can then be analyzed at a later time or re-analyzed to validate the data reproducibility. The requested equipment is a general-purpose tool that can store biological samples for an extensive period of time. Yeast samples harvested from a fuel alcohol production process will be stored in the requested freezer. These samples will be used to carry out whole-cell gene expression and protein expression profiling, attempting to probe yeast physiological variations under a dual osmotic (due to high sugar concentration) and inhibitory (due to high ethanol level) effect. Consequently, a control strategy can be developed to alleviate this adverse effect, toward the designing of a high ethanol-yield process for fuel alcohol industry.