Cryogenic freezer chest for sample storage

用于样品储存的低温冷冻箱

• 批准号: 345131-2007
• 负责人: Lin, YenHan
• 金额: $ 1.19万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=325628
• 关键词: Cryogenic; freezer; chest; sample; storage

The requested cryogenic freezer chest can store biological samples at -150 oC. No liquid nitrogen is required to keep stored samples at this temperature. At -150 oC, no ice re-crystallization will occur from within and outside samples and the biological activities of the sample are thus arrested. Samples can then be analyzed at a later time or re-analyzed to validate the data reproducibility. The requested equipment is a general-purpose tool that can store biological samples for an extensive period of time. Yeast samples harvested from a fuel alcohol production process will be stored in the requested freezer. These samples will be used to carry out whole-cell gene expression and protein expression profiling, attempting to probe yeast physiological variations under a dual osmotic (due to high sugar concentration) and inhibitory (due to high ethanol level) effect. Consequently, a control strategy can be developed to alleviate this adverse effect, toward the designing of a high ethanol-yield process for fuel alcohol industry.

要求的低温冷冻箱可以在-150 ℃下储存生物样品。在此温度下储存样品不需要液氮。在-150 ° C下，样品内外均不会发生冰重结晶，样品的生物活性因此被抑制。然后可以在稍后的时间分析样品或重新分析以验证数据再现性。所请求的设备是一种通用工具，可长时间储存生物样本。从燃料酒精生产过程中收获的酵母样品将储存在要求的冰柜中。这些样品将用于进行全细胞基因表达和蛋白质表达谱分析，试图探测双重渗透（由于高糖浓度）和抑制（由于高乙醇水平）作用下的酵母生理变化。因此，控制策略可以开发，以减轻这种不利影响，对燃料酒精工业的高乙醇收率的过程设计。