High Resolution Respirometer

准确测量代谢和线粒体功能对于理解基本的细胞、组织和全身功能至关重要。为了充分了解代谢和线粒体功能，使用高灵敏度呼吸测量设备进行分析至关重要。目前，滑铁卢大学还没有高分辨率的呼吸计。获得高分辨率呼吸计将使滑铁卢大学的研究人员能够研究啮齿动物和人类细胞器/细胞/组织的线粒体和代谢功能。该呼吸计将支持滑铁卢大学5个系（运动学、生物学、药剂学、公共卫生、系统设计工程）和3个学院（应用健康科学、科学和工程）的至少9名独立研究人员的NSERC和其他资助工作。此外，该设备将加强这些研究人员之间正在进行的合作，并有助于促进该小组与校园内其他用户之间的新合作。在这些实验室中，缺乏适当的呼吸计是充分和适当地了解细胞、代谢和线粒体功能的主要限制。所要求的高分辨率呼吸计将大大改进这些实验室目前使用的技术。这款呼吸计确实可以被视为一种“核心仪器”，因为它可以执行多种测量（氧气消耗、活性氧生成、离子水平、ATP生成、膜电位等）以及它将支持的基础研究范围(骨骼肌生物学、心血管生理学、神经学、免疫学、线粒体生物学、发育生物学、代谢、饮食/营养、运动生理学、药物发现、炎症、材料生物相容性)。获得这些设备也将促进本科生和研究生在这些实验室的培训，并为这些学生提供改进和加快他们的研究项目的机会。总的来说，这种能力将大大提高这些实验室的研究质量和影响，并促进本小组内部、整个大学和整个安大略省的新合作。

Accurately measuring metabolic and mitochondrial function is critical in understanding basic cellular, tissue, and whole body function. To fully understand metabolic and mitochondrial function it is crucial to perform analysis with high sensitive respirometry equipment. Currently, a high-resolution respirometer is not available at the University of Waterloo. Acquisition of a high resolution respirometer will allow researchers at the University of Waterloo to study mitochondrial and metabolic function in rodent and human organelles/cells/tissues. The respirometer will support the NSERC and other funded work of at least 9 independent researchers from 5 Departments (Kinesiology, Biology, Pharmacy, Public Health, Systems Design Engineering) and 3 Faculties (Applied Health Sciences, Science, and Engineering) at the University of Waterloo. In addition, this equipment will strengthen ongoing collaborations between these researchers, and help facilitate new collaborations between this group and other users across campus. The lack of an appropriate respirometer in these labs is a major constraint to fully and properly understanding cellular, metabolic, and mitochondrial function. The requested high-resolution respirometer will greatly improve upon the techniques currently employed in these laboratories. This respirometer can truly be viewed as a "core instrument" given it versatility in the measures it can perform (oxygen consumption, reactive oxygen species generation, ion levels, ATP production, membrane potential, etc.) as well as the range of basic research that it will support (skeletal muscle biology, cardiovascular physiology, neurology, immunology, mitochondrial biology, developmental biology, metabolism, diet/nutrition, exercise physiology, drug discovery, inflammation, materials biocompatibility). Acquiring this equipment will also facilitate training of undergraduate and graduate students in these laboratories, and provide these students with opportunities to improve and expedite their research projects. Overall, this capability will greatly enhance the quality and impact of research from these laboratories, and foster new collaborations within this group, across the University, and throughout Ontario.