Interaction between Staphylococcus aureus and bovine mammary epithelial cells

金黄色葡萄球菌（Staphylococcus aureus）是一种机会致病菌，可引起人类和动物的多种疾病。在乳品工业中，S.金黄色乳腺炎极难控制，并且通常发展成慢性感染，至少部分是由于细菌侵入上皮细胞，这可能有助于抵抗宿主的防御机制并降低抗生素治疗的功效。这项研究的总体目标是继续我以前的NSERC发现基金，并研究S。使用Clf-A作为模型系统，金黄色葡萄球菌附着并入侵牛乳腺上皮细胞。沙门氏菌对牛乳腺上皮细胞的侵袭。在原代和培养的乳腺上皮细胞以及小鼠模型系统中已经报道了金黄色葡萄球菌。粘附和随后的侵袭过程是建立慢性S。金黄色葡萄球菌乳房内感染。这两个过程是由一个大的，结构相关的，家庭的表面表达的蛋白质，包括凝集因子A介导。这项研究将集中在Clf-A上。

Staphylococcus aureus is an opportunistic pathogen that causes a variety of diseases in humans and animals. In dairy industry, S. aureus mastitis is extremely difficult to control and usually develops into a chronic infection, at least partially due to invasion of bacteria into epithelial cells, which may help resist defense mechanisms of the host and decrease the efficacy of antibiotic treatment. The global objective of this proposed research is to continue my previous NSERC discovery grant and to study the mechanisms by which surface adhesins of S. aureus attach to and invade bovine mammary epithelial cells, using Clf-A as a model system. Invasion of bovine mammary epithelial cells by S. aureus have been reported in primary and cultured mammary epithelial cells and in a mouse model system. Adherence and the subsequent invasion processes are the two most important and decisive prerequisites to the establishment of chronic S. aureus intramammary infections. These two processes are mediated by a large, structurally related, family of surface-expressed proteins including clumping factors A. This proposed research will focus on Clf-A.