Biogenesis and repair of photosynthetic memranes

植物和藻类通过光合作用从光中获得生长和繁殖所需的全部能量。在绿色组织的细胞内，光合作用发生在称为叶绿体的细胞器中，特别是通过称为类囊体的袋状囊泡网络进行。本提案描述了将解决类囊体膜如何产生的研究。拟议的研究将探索在类囊体膜产生的基础上的过程的空间组织。我们之前的研究表明，叶绿体在这方面要复杂得多，并且包含专门用于在类囊体膜中进行光合作用的蛋白质合成的室室。我们建议使用超现代的方法来深入表征这些膜，以了解它们的精确作用。这一结果将与加拿大工业生物技术的发展有关，因为藻类已经在食品工业中使用了几十年，并作为环境污染物的生物传感器。它们具有很高的潜力，可用于作为药物、疫苗和其他可销售产品的蛋白质的有效生产。实现这一潜力需要了解蛋白质是如何在叶绿体中产生的。

Plants and algae obtain all of their energy for growth and reproduction from light using a process called photosynthesis. Within the cells of green tissues, photosynthesis occurs in organelles called chloroplasts, and specifically with by a network of sack-like vesicles called thylakoids. This proposal describes research that would address how thylakoid membranes are generated. The proposed research will explore the spatial organization of the processes that underlie the generation of thylakoid membranes. Our previous research revealed that chloroplasts are much more complex in this regard, and contain compartments that are specialized in the synthesis of proteins that carry out photosynthesis in thylakoid membranes. We are proposing to characterize these membranes in depth using ultramodern approaches to understand their precise roles. The results will be relevant to the development of biotechnologies for Canadian industries because algae have been used in the food industry and as biosensors for environmental pollutants for decades. They have a high potential to be used for the efficient production of proteins as pharmaceuticals, vaccines, and other marketable products. Realization of this potential will require an understanding of how proteins are made in chloroplasts.