An ultracentrifuge and rotors to support multiple NSERC-funded programs

支持多个 NSERC 资助项目的超速离心机和转子

• 批准号: 472650-2015
• 负责人: Zerges, William
• 金额: $ 9.96万
• 依托单位: Concordia University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=564803
• 关键词: ultracentrifuge; rotors; support; multiple; NSERC

Ultracentrifugation is a standard technique in biological research used to identify and purify intracellular organelles, assemblies, biological membranes, and other molecules using a variety of different sophisticated methods. All of these methods use an ultracentrifuge to subject complex samples of biological material to extremely high centrifugal forces; on the order of 100,000 times the force of gravity. These samples are held in rotors which are designed to hold tubes of the proper size and configuration. Seven research groups in the Biology Department of Concordia University routinely require ultracentrifugation. Consequently, the single ultracentrifuge in the department is heavily used; running nearly continuously on 95% of weekdays (and nights) and 50% of weekends throughout the year. The few rotors are decades-old and shared with other departments. Therefore, there is an urgent need for a second common-use ultracentrifuge and new rotors in the Biology Department.

超离心是生物学研究中的标准技术，用于使用各种不同的复杂方法鉴定和纯化细胞内细胞器、组装体、生物膜和其他分子。所有这些方法都使用超离心力使复杂的生物材料样品受到极高的离心力;大约是重力的10万倍。将这些样品固定在转子中，转子设计用于固定适当尺寸和配置的试管。康考迪亚大学生物系的七个研究小组经常需要超浓缩。因此，该部门的单一超级计算机被大量使用;全年95%的工作日（和夜晚）和50%的周末几乎连续运行。几个转子有几十年的历史，与其他部门共享。因此，生物系迫切需要第二种通用的超离心泵和新转子。