Localization of translation and non-translated mRNAs; functions, mechanisms and novel compartments
翻译和非翻译 mRNA 的定位;
基本信息
- 批准号:RGPIN-2019-07009
- 负责人:
- 金额:$ 3.64万
- 依托单位:
- 依托单位国家:加拿大
- 项目类别:Discovery Grants Program - Individual
- 财政年份:2020
- 资助国家:加拿大
- 起止时间:2020-01-01 至 2021-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Plants and algae obtain energy for growth and reproduction from light using a process called photosynthesis. Photosynthesis occurs in subcellular organelles called chloroplasts. Our previous research revealed that chloroplasts contain compartments for the synthesis of proteins and RNA quality control. We are analyzing these compartments as an avenue to understand biogenesis and quality control in this organelle and, we hope, reveal general principles of Cell Biology.
One goal of this research program is to understand how the membranes that make up thylakoid vesicles within chloroplasts are made. Thylakoid vesicles carry out the light-driven reactions of photosynthesis. We have identified a special region of the membranous envelope that encloses the chloroplast where proteins and chlorophyll of the thylakoids are generated. We show for the first time that the ribosomes of the cell's cytoplasm are synthesizing proteins in direct contact with the chloroplast. This has been show for most other organelles, but not for chloroplasts. Characterization of the composition of this novel biogenic domain of the chloroplast envelope will lead to new insights regarding the long-standing mystery regarding the developmental origins of thylakoid membranes and how they arise from the chloroplast envelope.
Another goal is to characterize a compartment within the chloroplast that handles quality control of RNA. RNA is an important molecule in the transfer of genetic information from the gene to generate functional proteins. Chloroplasts have their own genome and gene expression system because they evolved from a free-living photosynthetic bacterium which took up residence in the primordial plant cell some 1-2 billion year ago. Since, chloroplasts have retained a downsized genome and gene expression system, including RNA. We identified and study structures are called “chloroplast stress granules” which handle RNA that has just left the protein synthesis machinery and is not being translated. These chloroplast stress granules are similar to stress granules found in all eukaryotic cells examined and even in bacteria called stress granules. We will characterize chloroplast stress granules to learn about how this organelle handles RNA quality control and, we hope, provide general insights into stress granule functions in all organisms.
In summary, the results will be relevant to the development of biotechnologies for Canadian industries because algae are used in the food industry and as biosensors for environmental pollutants for decades. Algae are being engineered to efficiently produce high-value molecules at industrial scales; e.g. pharmaceuticals, vaccines, and food supplements. Realization of this potential will benefit from our research results regarding how proteins are synthesized in chloroplasts. The results will also contribute to the basic knowledge that will be required for the biotechnological improvement of crop plants in agriculture.
植物和藻类通过光合作用从光中获取生长和繁殖所需的能量。光合作用发生在称为叶绿体的亚细胞细胞器中。我们之前的研究表明,叶绿体中含有用于蛋白质合成和RNA质量控制的室室。我们正在分析这些区室,作为了解细胞器生物发生和质量控制的途径,我们希望揭示细胞生物学的一般原理。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
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专利数量(0)
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Zerges, William其他文献
Stress induces the assembly of RNA granules in the chloroplast of Chlamydomonas reinhardtii
- DOI:
10.1083/jcb.200805125 - 发表时间:
2008-08-25 - 期刊:
- 影响因子:7.8
- 作者:
Uniacke, James;Zerges, William - 通讯作者:
Zerges, William
Chloroplast protein targeting involves localized translation in Chlamydomonas
- DOI:
10.1073/pnas.0811268106 - 发表时间:
2009-02-03 - 期刊:
- 影响因子:11.1
- 作者:
Uniacke, James;Zerges, William - 通讯作者:
Zerges, William
Biogenic membranes of the chloroplast in Chlamydomonas reinhardtii
- DOI:
10.1073/pnas.1209860109 - 发表时间:
2012-11-20 - 期刊:
- 影响因子:11.1
- 作者:
Schottkowski, Marco;Peters, Matthew;Zerges, William - 通讯作者:
Zerges, William
Photosystem II assembly and repair are differentially localized in Chlamydomonas
- DOI:
10.1105/tpc.107.054882 - 发表时间:
2007-11-01 - 期刊:
- 影响因子:11.6
- 作者:
Uniacke, James;Zerges, William - 通讯作者:
Zerges, William
Zerges, William的其他文献
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{{ truncateString('Zerges, William', 18)}}的其他基金
Localization of translation and non-translated mRNAs; functions, mechanisms and novel compartments
翻译和非翻译 mRNA 的定位;
- 批准号:
RGPIN-2019-07009 - 财政年份:2022
- 资助金额:
$ 3.64万 - 项目类别:
Discovery Grants Program - Individual
Localization of translation and non-translated mRNAs; functions, mechanisms and novel compartments
翻译和非翻译 mRNA 的定位;
- 批准号:
RGPIN-2019-07009 - 财政年份:2021
- 资助金额:
$ 3.64万 - 项目类别:
Discovery Grants Program - Individual
Localization of translation and non-translated mRNAs; functions, mechanisms and novel compartments
翻译和非翻译 mRNA 的定位;
- 批准号:
RGPIN-2019-07009 - 财政年份:2019
- 资助金额:
$ 3.64万 - 项目类别:
Discovery Grants Program - Individual
Biogenesis and repair of photosynthetic memranes
光合膜的生物发生和修复
- 批准号:
217566-2013 - 财政年份:2018
- 资助金额:
$ 3.64万 - 项目类别:
Discovery Grants Program - Individual
Biogenesis and repair of photosynthetic memranes
光合膜的生物发生和修复
- 批准号:
217566-2013 - 财政年份:2017
- 资助金额:
$ 3.64万 - 项目类别:
Discovery Grants Program - Individual
Biogenesis and repair of photosynthetic memranes
光合膜的生物发生和修复
- 批准号:
217566-2013 - 财政年份:2016
- 资助金额:
$ 3.64万 - 项目类别:
Discovery Grants Program - Individual
Biogenesis and repair of photosynthetic memranes
光合膜的生物发生和修复
- 批准号:
445984-2013 - 财政年份:2015
- 资助金额:
$ 3.64万 - 项目类别:
Discovery Grants Program - Accelerator Supplements
Biogenesis and repair of photosynthetic memranes
光合膜的生物发生和修复
- 批准号:
217566-2013 - 财政年份:2015
- 资助金额:
$ 3.64万 - 项目类别:
Discovery Grants Program - Individual
An ultracentrifuge and rotors to support multiple NSERC-funded programs
支持多个 NSERC 资助项目的超速离心机和转子
- 批准号:
472650-2015 - 财政年份:2014
- 资助金额:
$ 3.64万 - 项目类别:
Research Tools and Instruments - Category 1 (<$150,000)
Biogenesis and repair of photosynthetic memranes
光合膜的生物发生和修复
- 批准号:
217566-2013 - 财政年份:2014
- 资助金额:
$ 3.64万 - 项目类别:
Discovery Grants Program - Individual
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