Development of a scalable in vitro sialylation process for butyrylcholinesterase

有机磷酸盐，包括沙林毒气和VX神经毒剂，不可逆地结合乙酰胆碱酯酶（BChE），并在几分钟内导致人体瘫痪和死亡。目前的方法是从人血清中分离出BChE，不能以合理的成本生产出加拿大和美国军队预防性和急性使用所需的数量。然而，我们的合作伙伴PlantForm公司可以使用其基于植物的生物反应器生产克数量的BChE。在我们与PlantForm的合作中，我们将在植物生产的BChe上使用糖的生化工程，这将使蛋白质在体内停留更长时间，从而保护人们免受这些致命神经毒剂的侵害。

Organophosphates, including Sarin gas and VX nerve agent, irreversibly bind acetylcholinesterase (BChE) and induce human paralysis and death within minutes. Current methods isolate BChE from human serum, which cannot produce the quantity required at reasonable costs for prophylactic and acute use in the Canadian and US military. However, our collaborator PlantForm Corporation can produce gram quantities of BChE using its plant based bio-reactors. In our collaboration with PlantForm will use biochemical engineering of sugars on the plant produced BChe which will then enable the protein to stay in the body longer thus protecting people longer from these deadly nerve agents.