Tetrad Dissection Microscope for Yeast Genetics Analyses

因为酵母很容易在实验室中生长和遗传操作，生物学家在过去几十年里广泛使用酵母，在细胞过程中取得了重要发现，其中许多是人类和其他真核生物共同的。在后基因组时代，通过广泛而全面的全基因组遗传分析，酵母在快速推进我们对生物如何协调基因表达和应对压力的知识方面发挥了核心作用。约克大学生物系现在有两个实验室使用酵母作为他们的主要模式生物。为了充分利用这种生物提供的潜力，并在大学开发一个设备齐全的酵母遗传学设施，我们正在申请资金购买一台四分体解剖显微镜。该仪器是实验室研究酵母必不可少的工具，用于产生特定的突变菌株，检查这些菌株的生长表型，并发现揭示生物途径之间关系的新的遗传相互作用。

Because it is easy to grow in the lab and manipulate genetically, biologists have used yeast extensively in the past several decades to make important discoveries about cellular processes, many of which are common to humans and other eukaryotic organisms. In the post-genomic era, through vast and comprehensive genome-wide genetic analyses, yeast has played a central role in rapidly advancing our knowledge of how organisms coordinate the expression of their genes and respond to stress. The Department of Biology at York University now has two labs that use yeast as their primary model organism. To fully benefit from the potential that working with this organism provides, and towards developing a fully-equipped yeast genetics facility at the university, we are requesting funds to acquire a tetrad dissection microscope. This instrument, which is an indispensable tool for labs working with yeast, is used for generating specific mutant strains, for examining the growth phenotypes of these strains, and for uncovering novel genetic interactions that reveal relationships between biological pathways.