Plant transcriptomic and metabolomic core platform

植物对环境做出反应，并调节其基因的转录，以最大限度地促进生存和生长。转录改变的结果导致表达各种蛋白质来制造与植物环境中普遍存在的情况相适应的化合物。这种化合物被称为代谢物，代表着植物中存在的数百万分子的强大储存库。提案中要求的设备旨在实现以下两个目标：1)监测植物基因表达；2)量化植物产生的各种代谢物的数量和多样性。请求设备的两个实验室由年轻教授领导(分别于2012年和2013年受聘)，他们的研究项目由NSERC(发现和参与)、FRQNT(新切切尔)和一个机构研究主席(UQTR)资助。虽然两个PI都成功地获得了运营拨款，但他们(和他们的部门)缺乏足够的设备来监测基因表达和代谢组变化，尽管这些简单的设备是他们发现研究计划的基础。这两个研究项目都使用基于研究的系统生物学，例如整合转录组和代谢组数据来阐明不同的植物过程。具体地说，Germain博士的研究项目侧重于植物对生物营养病原体的免疫反应，这一过程涉及几个受植物激素水杨酸调控的基因，而水杨酸的水平只能通过高效液相色谱法进行监测。Desgagné-Penix博士的研究旨在阐明生化途径，特别是在非模式植物中异喹啉生物碱的生物合成(例如，治疗阿尔茨海默病的加兰他明；抗癌药物纳西拉辛；以及抗菌化合物肌氨酸)。要成功地阐明这种途径，需要比较产生(或不产生)特定代谢物生物碱的植物之间的基因表达(本身由高效液相色谱监测)。因此，正如他们的发现基金中所概述的那样，基因表达和代谢物图谱对他们的研究计划至关重要。

Plants react to their environment and modulate the transcription of their genes to maximize survival and growth. The outcomes of transcriptomic changes results in various proteins being expressed to manufacture the compounds befitting the situation prevailing in the plant environment. Such compounds are termed metabolites and represent a formidable reservoir of millions of molecules present in plants. The equipment requested in the proposal aims the two following goals; 1) monitor plant gene expression 2) quantify the amount and diversity of various metabolites produced by plants. The two laboratories requesting the equipment are led by young professors (hired in 2012 and 2013 respectively) whose research programs are funded by NSERC (Discovery and Engage), FRQNT (Nouveau chercheur) and an institutional research chair (UQTR). While both PI have been successful at obtaining operating grant, they (and their department) lack the adequate equipment to monitor gene expression and metabolome variation despite the fact that these simple equipment are at the basis of their Discovery research program. Both research programs use research-based systems biology such as the integration of transcriptome and metabolome data to elucidate different plant processes. Specifically, Dr. Germain's research program focuses on plant immune response to biotrophic pathogen a process involving the implication of several genes regulated by the phytohormone salicylic acid whose level can only be monitored by HPLC. Dr. Desgagné-Penix research aims the elucidation of biochemical pathways, specifically the biosynthesis of isoquinoline alkaloids (e.g. galanthamine, an Alzheimer's disease drug treatment; narciclasine, an anti-cancer agent; and crinine, an antimicrobial compound) in non-model plants. Successful elucidation of such pathways requires the comparison of gene expression between plant producing (or not) the given metabolite alkaloid (itself monitored by HPLC). Hence, as outlined in their Discovery grant, gene expression and metabolite profiling is crucial to their research program.